Antibody data
- Antibody Data
- Antigen structure
- References [1]
- Comments [0]
- Validations
- Western blot [2]
- Immunohistochemistry [1]
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Validation data
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- Product number
- PA5-36521 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- Aquaporin 4 Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Synthetic peptide
- Description
- This antibody detects endogenous protein at a molecular weight of 35 kDa. Purity is >95% by SDS-PAGE.
- Reactivity
- Human, Mouse, Rat
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 100 µL
- Concentration
- 1 mg/mL
- Storage
- Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
Submitted references Meningeal Lymphangiogenesis and Enhanced Glymphatic Activity in Mice with Chronically Implanted EEG Electrodes.
Hauglund NL, Kusk P, Kornum BR, Nedergaard M
The Journal of neuroscience : the official journal of the Society for Neuroscience 2020 Mar 11;40(11):2371-2380
The Journal of neuroscience : the official journal of the Society for Neuroscience 2020 Mar 11;40(11):2371-2380
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of Aquaporin-4 using Aquaporin-4 polyclonal antibody (Product # PA5-36521) at a dilution of 1:500. Lane 1: Hela cell lysate, Lane 2: Jurkat cell lysate, Lane 3: PC12 cell lysate, Lane 4: sp2/0 cell lysate.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot was performed using Aquaporin 4 Polyclonal Antibody (Product # PA5-36521) and a~48 kDa band corresponding to AQP4 was observed in A549, A431, U87MG, Mouse Brain and Rat Brain; and was low or absent in Mouse Ileum, Mouse Liver, Rat Ileum and Rat Liver. Whole cell extracts (30 µg lysate) of A549 (Lane 1), A431 (Lane 2), U87MG (Lane 3) and tissues Mouse Skeletal Muscle (Lane 4), Mouse Brain (Lane 5), Mouse Ileum (Lane 6), Mouse Liver (Lane 7), Rat Skeletal Muscle (Lane 8) Rat Brain (Lane 9), Rat Ileum (Lane 10) and Rat Liver (Lane 11) were electrophoresed using NuPAGE® 4-12 % Bis-Tris gel (Product # NP0322BOX). Resolved proteins were then transferred onto a nitrocellulose membrane (Product # IB23001) by iBlot® 2 Dry Blotting System (Product # IB21001). The blot was probed with the primary antibody (1:1000 dilution) and detected by chemiluminescence with Goat anti-Rabbit IgG (H+L) Superclonal™ Recombinant Secondary Antibody, HRP (Product # A27036, 1:4000 dilution) using the iBright FL 1000 (Product # A32752). Chemiluminescent detection was performed using Novex® ECL Chemiluminescent Substrate Reagent Kit (Product # WP20005). An uncharacterized band at ~38 kDa was found in Mouse Liver.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemical analysis of Aquaporin-4 in paraffin-embedded human lung carcinoma using Aquaporin-4 polyclonal antibody (Product # PA5-36521).