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- Antibody Data
- Antigen structure
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- Validations
- Western blot [1]
- Immunohistochemistry [5]
- Other assay [1]
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- Product number
- MA5-24587 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- Aquaporin 4 Monoclonal Antibody (CL0178)
- Antibody type
- Monoclonal
- Antigen
- Recombinant full-length protein
- Description
- Immunogen sequence: CPDVEFKRRF KEAFSKAAQQ TKGSYMEVED NRSQVETDDL ILKPGVVHVI DVDRGEEKKG KDQSGEVLSS V
- Antibody clone number
- CL0178
- Concentration
- 1.00 mg/mL
Submitted references Expression of IL-20 Receptor Subunit β Is Linked to EAE Neuropathology and CNS Neuroinflammation.
Dayton JR, Yuan Y, Pacumio LP, Dorflinger BG, Yoo SC, Olson MJ, Hernández-Suárez SI, McMahon MM, Cruz-Orengo L
Frontiers in cellular neuroscience 2021;15:683687
Frontiers in cellular neuroscience 2021;15:683687
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of Aquaporin 4 in Lane1: Marker (kDa), Lane 2: Human cell line U-251 MG Samples were probed using a Aquaporin 4 Monoclonal Antibody (Product # MA5-24587).
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemical staining of Aquaporin 4 in human cerebral cortex and liver tissues using Aquaporin 4 Monoclonal Antibody (CL0178) (Product # MA5-24587). Corresponding AQP4 RNA-seq data are presented for the same tissues.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemical staining of Aquaporin 4 in human cerebral cortex using Aquaporin 4 Monoclonal Antibody (CL0178) (Product # MA5-24587) shows moderate to strong membranous positivity in astrocytes.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemical staining of Aquaporin 4 in human cerebellum using Aquaporin 4 Monoclonal Antibody (CL0178) (Product # MA5-24587) shows strong positivity in neuropil, mainly in the molecular layer.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemical staining of Aquaporin 4 in human stomach using Aquaporin 4 Monoclonal Antibody (CL0178) (Product # MA5-24587) shows moderate to strong membranous positivity in glandular cells.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemical staining of Aquaporin 4 in human liver using Aquaporin 4 Monoclonal Antibody (CL0178) (Product # MA5-24587) shows no positivity in hepatocytes as expected.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- FIGURE 5 IL-20 receptor subunit beta expression on rhesus macaque CNS microvessels. CNS region-specific microvessels were isolated from macaque tissue, n = 3, to elucidate IL-20RB protein expression by immunolabeling for VEGFR-2 (white, endothelial marker), IL-20RB (red), AQP4 (green, astrocytic end-feet marker), and DAPI (blue), scale bar = 10 mum (A) . VEGFR-2 colocalized with IL-20RB confirming endothelial cells expression of IL-20RB whereas AQP4 appears to envelop both. Histogram analysis was performed to 50 microvessels per specimen and CNS region to determine microvessels diameter and AUI for IL-20RB expression (representative AUI vs. axis plot, B ). One-way ANOVA analysis followed by Sidak's post hoc test showed no statistical significance between AUC means for the different CNS regions (mean +- SD cortex: 6.4 x 10 -4 +- 2.9 x 10 -5 , cerebellum: 6.0 x 10 -4 +- 7.3 x 10 -5 , brainstem: 6.4 x 10 -4 +- 2.5 x 10 -5 and spinal cord: 7.1 x 10 -4 +- 6.7 x 10 -5 , C ). Results are shown as mean +- SD.
