Antibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [2]
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Validation data
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- Product number
- PA5-77709 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- Aquaporin 4 Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Other
- Description
- For reconstitution, we recommend adding 100 µL distilled water to a final antibody concentration of about 1 mg/mL. To use this carrier-free antibody for conjugation experiments, we strongly recommend performing another round of desalting. (Zeba Spin Desalting Columns, 7KMWCO, 0.5 mL, Product # 89882)
- Reactivity
- Human, Mouse, Rat
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 50 µL
- Concentration
- 0.8 mg/mL
- Storage
- -20°C
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of rat brain membranes with Aquaporin 4 polyclonal antibody (Product # PA5-77709) using a dilution of 1:1000.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot was performed using Aquaporin 4 Polyclonal Antibody (Product # PA5-77709) and ~45 kDa band corresponding to AQP4 was observed in Mouse Skeletal Muscle, Mouse Brain, Rat Skeletal Muscle and Rat Brain; but was absent in Mouse Ileum, Mouse Liver, Rat Ileum and Rat Liver. Tissue extracts (30 µg lysate) of Mouse Skeletal Muscle (Lane 1), Mouse Brain (Lane 2), Mouse Ileum (Lane 3), Mouse Liver (Lane 4), Rat Skeletal Muscle (Lane 5), Rat Brain (Lane 6), Rat Ileum (Lane 7) and Rat Liver (Lane 8) were electrophoresed using NuPAGE® 4-12 % Bis-Tris gel (Product # NP0322BOX). Resolved proteins were then transferred onto a nitrocellulose membrane (Product # IB23001) by iBlot® 2 Dry Blotting System (Product # IB21001). The blot was probed with the primary antibody (1:500 dilution) and detected by chemiluminescence with Goat anti-Rabbit IgG (H+L) Superclonal™ Recombinant Secondary Antibody, HRP (Product # A27036, 1:4000 dilution) using the iBright FL 1000 (Product # A32752). Chemiluminescent detection was performed using Novex® ECL Chemiluminescent Substrate Reagent Kit (Product # WP20005). Uncharacterized band (*) were observed in Mouse Skeletal Muscle.