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Western blot
ImmunocytochemistryAntibody data
- Antibody Data
- Antigen structure
- References [1]
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- Validations
- Western blot [2]
- Immunoprecipitation [1]
- Immunohistochemistry [1]
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- Product number
- NBP2-19461 - Provider product page
- Provider
- Novus Biologicals
- Proper citation
- Novus Cat#NBP2-19461, RRID:AB_2687574
- Product name
- Rabbit Polyclonal NAA10 Antibody
- Antibody type
- Polyclonal
- Description
- Immunogen affinity purified.
- Reactivity
- Human, Mouse
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 0.1 ml
- Storage
- Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
Submitted references The Mammalian Ribo-interactome Reveals Ribosome Functional Diversity and Heterogeneity.
Simsek D, Tiu GC, Flynn RA, Byeon GW, Leppek K, Xu AF, Chang HY, Barna M
Cell 2017 Jun 1;169(6):1051-1065.e18
Cell 2017 Jun 1;169(6):1051-1065.e18
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Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: NAA10 Antibody [NBP2-19461] - Sample (30 ug of whole cell lysate) A: H1299 transfected scramble siRNA B: H1299 transfected ARDIA siRNA 12% SDS PAGE gel, diluted at 1:5000.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: NAA10 Antibody [NBP2-19461] - Various whole cell extracts (30 ug) were separated by 12% SDS-PAGE, and blotted with ARD1A antibody diluted by 1:1000. The HRP-conjugated anti-rabbit IgG antibody (NBP2-19301) was used to detect the primary antibody.
Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunoprecipitation: NAA10 Antibody [NBP2-19461] - Immunoprecipitation of ARD1A protein from HeLa whole cell extracts using 5 ug of ARD1A antibody. Western blot analysis was performed using ARD1A antibody. EasyBlot anti-Rabbit IgG was used as a secondary reagent.
Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunohistochemistry-Paraffin: NAA10 Antibody [NBP2-19461] - Immunohistochemical analysis of paraffin-embedded PC13 xenograft, using antibody at 1:500 dilution.
