Explore
Validate
Learn
Western blot
ImmunoprecipitationAntibody data
- Antibody Data
- Antigen structure
- References [1]
- Comments [0]
- Validations
- Western blot [2]
- Immunocytochemistry [1]
- Immunohistochemistry [1]
Submit
Validation data
Reference
Comment
Report error
- Product number
- PA5-32236 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- ARD1A Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Recombinant full-length protein
- Description
- Recommended positive controls: H1299 transfected scramble siRNA, H1299 transfected ARDIA siRNA, Neuro2A, C8D30, NIH-3T3, Raw264.7, C2C12. Predicted reactivity: Mouse (96%), Rat (96%), Xenopus laevis (83%), Rabbit (97%), Bovine (97%). Store product as a concentrated solution. Centrifuge briefly prior to opening the vial.
- Reactivity
- Human, Mouse
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 100 µL
- Concentration
- 0.73 mg/mL
- Storage
- Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
Submitted references Overlap of NatA and IAP substrates implicates N-terminal acetylation in protein stabilization.
Mueller F, Friese A, Pathe C, da Silva RC, Rodriguez KB, Musacchio A, Bange T
Science advances 2021 Jan;7(3)
Science advances 2021 Jan;7(3)
No comments: Submit comment
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western Blot using ARD1A Polyclonal Antibody (Product # PA5-32236). Sample (30 µg of whole cell lysate). Lane A: H1299 transfected scramble siRNA. Lane B: H1299 transfected ARDIA siRNA. 12% SDS PAGE. ARD1A Polyclonal Antibody (Product # PA5-32236) diluted at 1:5,000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- ARD1A Polyclonal Antibody detects ARD1A protein by western blot analysis. Various whole cell extracts (30 µg) were separated by 12% SDS-PAGE, and blotted with ARD1A Polyclonal Antibody (Product # PA5-32236) diluted by 1:1,000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- ARD1A Polyclonal Antibody detects NAA10 protein at Cytoplasm by immunofluorescent analysis. Sample: HeLa cells were fixed in -20ºC 100% MeOH for 5 min. Green: NAA10 protein stained by ARD1A Polyclonal Antibody (Product # PA5-32236) diluted at 1:500. Blue: Hoechst 33343 staining.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemical analysis of paraffin-embedded PC13 xenograft, using ARD1A (Product # PA5-32236) antibody at 1:500 dilution. Antigen Retrieval: EDTA based buffer, pH 8.0, 15 min.
