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- Antibody Data
- Antigen structure
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- Validations
- Western blot [2]
- Immunohistochemistry [2]
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- Product number
- OAAB03675 - Provider product page
- Provider
- Aviva Systems Biology
- Product name
- ETHE1 antibody - center region
- Antibody type
- Polyclonal
- Reactivity
- Human, Mouse
- Host
- Rabbit
- Vial size
- 400ul
- Storage
- Maintain refrigerated at 2-8 deg C for up to 6 months. For long term storage store at -20 deg C in small aliquots to prevent freeze-thaw cycles.
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Supportive validation
- Submitted by
- Aviva Systems Biology (provider)
- Main image
- Experimental details
- Western blot analysis of ETHE1 (arrow) using rabbit polyclonal ETHE1 Antibody (Center) either nontransfected (Lane 1) or lysates transiently transfected transfected (Lane 2) with the ETHE1 gene.
- Sample type
- lysatestransiently transfected with the ETHE1 gene.
- Primary Ab dilution
- 1.0 µg/mL
- Protocol
- Protocol
- Submitted by
- Aviva Systems Biology (provider)
- Main image
- Experimental details
- Western blot analysis of ETHE1 antibody (Center) in mouse kidney tissue lysates (35ug/lane). ETHE1 (arrow) was detected using the purified Pab.
- Sample type
- mouse kidney tissue lysates
- Primary Ab dilution
- 1.0 µg/mL
- Protocol
- Protocol
Supportive validation
- Submitted by
- Aviva Systems Biology (provider)
- Main image
- Experimental details
- Flow cytometric analysis of 293 cells using ETHE1 Antibody (Center)(bottom histogram) compared to a negative control cell (top histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
- Sample type
- Flow cytometric analysis of 293 cells using ETHE1 Antibody (Center)(bottom histogram) compared to a negative control cell (top histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
- Primary Ab dilution
- 1.0 µg/mL
- Protocol
- Protocol
- Submitted by
- Aviva Systems Biology (provider)
- Main image
- Experimental details
- ETHE1 Antibody (Center) immunohistochemistry analysis in formalin fixed and paraffin embedded human colon carcinoma followed by peroxidase conjugation of the secondary antibody and DAB staining. This data demonstrates the use of the ETHE1 Antibody (Center) for immunohistochemistry. Clinical relevance has not been evaluated.
- Sample type
- human colon carcinoma
- Primary Ab dilution
- 1.0 µg/mL
- Protocol
- Protocol
