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Western blotAntibody data
- Antibody Data
- Antigen structure
- References [1]
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- Validations
- Western blot [2]
- Immunocytochemistry [1]
- Immunohistochemistry [3]
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- Product number
- PA5-56040 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- ETHE1 Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Recombinant full-length protein
- Description
- Immunogen sequence: RTDFQQGCAK TLYHSVHEKI FTLPGDCLIY PAHDYHGFTV STVEEERTLN PRLTLSCEEF VKIMGNLNLP KPQQIDFAVP ANMRCG Highest antigen sequence identity to the following orthologs: Mouse - 93%, Rat - 92%.
- Reactivity
- Human, Mouse, Rat
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 100 µL
- Concentration
- 0.30 mg/mL
- Storage
- Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
Submitted references Hydrogen sulfide, oxygen, and calcium regulation in developing human airway smooth muscle.
Bartman CM, Schiliro M, Helan M, Prakash YS, Linden D, Pabelick C
FASEB journal : official publication of the Federation of American Societies for Experimental Biology 2020 Sep;34(9):12991-13004
FASEB journal : official publication of the Federation of American Societies for Experimental Biology 2020 Sep;34(9):12991-13004
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of ETHE1 in Lane 1: Marker (kDa) 230, 130, 95, 72, 56, 36, 28, 17, 11; Lane 2: Human cell line RT-4; Lane 3: Human cell line U-251MG sp; Lane 4: Human plasma (IgG/HSA depleted); Lane 5: Human liver tissue.; Lane 6: Human tonsil tissue. tissue. Samples were probed using an ETHE1 Polyclonal Antibody (Product # PA5-56040).
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of ETHE1 in Lane 1: NIH-3T3 cell lysate (Mouse embryonic fibroblast cells); Lane 2: NBT-II cell lysate (Rat Wistar bladder tumour cells). Samples were probed using an ETHE1 Polyclonal Antibody (Product # PA5-56040).
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunofluorescent staining of ETHE1 in human cell line U-2 OS shows positivity in mitochondria & nucleus but excluded from the nucleoli. Samples were probed using an ETHE1 Polyclonal Antibody (Product # PA5-56040).
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemical staining of ETHE1 in human rectum using an ETHE1 Polyclonal Antibody (Product # PA5-56040) shows strong cytoplasmic positivity in glandular cells.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemical staining of ETHE1 in human testis using ETHE1 Polyclonal Antibody (Product # PA5-56040) shows low expression as expected.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemical staining of ETHE1 in human colon using ETHE1 Polyclonal Antibody (Product # PA5-56040) shows high expression.
