PA5-56040

antibody from Invitrogen Antibodies

Targeting: ETHE1 HSCO, YF13H12

Western blot (Recommended by provider)

Immunocytochemistry (Supportive data in Antibodypedia)

Immunohistochemistry (Supportive data in Antibodypedia)

Antibody Data

Product number

PA5-56040

Provider

Invitrogen Antibodies

Product name

ETHE1 Polyclonal Antibody

Antibody type

Polyclonal

Antigen

Recombinant protein fragment

Description

Immunogen sequence: RTDFQQGCAK TLYHSVHEKI FTLPGDCLIY PAHDYHGFTV STVEEERTLN PRLTLSCEEF VKIMGNLNLP KPQQIDFAVP ANMRCG Highest antigen sequence identity to the following orthologs: Mouse - 93%, Rat - 92%.

Reactivity

Human, Mouse, Rat

Host

Rabbit

Isotype

IgG

Vial size

100 μL

Concentration

0.30 mg/mL

Storage

Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

References

Submitted references

Hydrogen sulfide, oxygen, and calcium regulation in developing human airway smooth muscle.

Bartman CM, Schiliro M, Helan M, Prakash YS, Linden D, Pabelick C
FASEB journal : official publication of the Federation of American Societies for Experimental Biology 2020 Sep;34(9):12991-13004

Immunocytochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunofluorescent staining of ETHE1 in human cell line U-2 OS shows positivity in mitochondria & nucleus but excluded from the nucleoli. Samples were probed using an ETHE1 Polyclonal Antibody (Product # PA5-56040).

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunofluorecent analysis of ETHE1 in human cell line U-2 OS using ETHE1 Polyclonal Antibody (Product # PA5-56040). Staining shows localization to nucleoplasm and mitochondria.

Immunohistochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemical analysis of ETHE1 in human colon using ETHE1 Polyclonal Antibody (Product # PA5-56040) shows strong granular cytoplasmic positivity in glandular cells.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemical staining of ETHE1 in human testis using ETHE1 Polyclonal Antibody (Product # PA5-56040) shows low expression as expected.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemical staining of ETHE1 in human colon using ETHE1 Polyclonal Antibody (Product # PA5-56040) shows high expression.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemical analysis of ETHE1 in human skeletal muscle using ETHE1 Polyclonal Antibody (Product # PA5-56040) shows no positivity in myocytes as expected.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemical staining of ETHE1 in human rectum using an ETHE1 Polyclonal Antibody (Product # PA5-56040) shows strong cytoplasmic positivity in glandular cells.