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Western blot
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ImmunoprecipitationAntibody data
- Antibody Data
- Antigen structure
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- Validations
- Western blot [2]
- Immunohistochemistry [3]
- Other assay [1]
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- Product number
- PA5-80601 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- ETHE1 Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Recombinant full-length protein
- Description
- This product is preservative free. It is recommended to add sodium azide to avoid contamination (final concentration 0.05%-0.1%). This antibody has specificity for Human ETHE1/HSCO.
- Reactivity
- Human
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 100 µL
- Concentration
- 1 mg/mL
- Storage
- Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of ETHE1 in Lane A: HepG2 Whole Cell Lysate (30 µg), Lane B: HCT116 Whole Cell Lysate (30 µg) . Samples were probed using a ETHE1 Polyclonal Antibody (Product # PA5-80601) at a 1:500 dilution, followed by a Goat Anti-Rabbit IgG (H+L), Dylight 800 Secondary Antibody at a 1:10000 dilution. Western blot was performed under reducing conditions. Predicted band size:28 kDa. Observed band size:25 kDa.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western Blot using ETHE1 Polyclonal Antibody (Product # PA5-80601) at 1:500 dilution. Lane A: HepG2 Whole Cell Lysate, Lane B: HCT116 Whole Cell Lysate. Lysates/proteins at 30 μg per lane. Secondary Goat Anti-Rabbit IgG H&L (DyLight™ 800) at 1:10,000 dilution. Developed using the Odyssey technique. Performed under reducing conditions. Predicted band size: 28 kDa. Observed band size: 25 kDa. (We are unsure of the identity of these extra bands).
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemical staining of human ETHE1 in human appendix with ETHE1 Polyclonal Antibody (Product # PA5-80601, 1:10,000, formalin-fixed paraffin embedded sections).
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemical staining of human ETHE1 in human colon carcinoma with ETHE1 Polyclonal Antibody (Product # PA5-80601, 1:10,000, formalin-fixed paraffin embedded sections).
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemical staining of human ETHE1 in human pancreas with ETHE1 Polyclonal Antibody (Product # PA5-80601, 1:10,000, formalin-fixed paraffin embedded sections).
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- ETHE1 Immunoprecipitation using: Lane A: 0.5 mg Hela Whole Cell Lysate, Lane B: 0.5 mg HepG2 Whole Cell Lysate, Lane C: 0.5 mg Jurkat Whole Cell Lysate 2 µL with ETHE1 Polyclonal Antibody (Product # PA5-80601) and 15 µL of 50 % Protein G agarose. Primary antibody: ETHE1 Polyclonal Antibody, at 1:100 dilution. Secondary antibody: Dylight 800-labeled antibody to rabbit IgG (H+L), at 1:5,000 dilution. Developed using the Odyssey technique. Performed under reducing conditions. Predicted band size: 28 kDa. Observed band size: 28 kDa.
