Antibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [1]
- Immunocytochemistry [2]
- Immunohistochemistry [2]
- Flow cytometry [1]
Submit
Validation data
Reference
Comment
Report error
- Product number
- PA5-86045 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- MYL2 Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Synthetic peptide
- Reactivity
- Human, Mouse, Rat
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 100 µL
- Concentration
- 1 mg/mL
- Storage
- Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
No comments: Submit comment
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of MYL2 in different lysates using a Polyclonal antibody (Product #PA5-86045) at a dilution of 1:500. Positive control: Lane1: Mouse skeletal muscle, Lane 2: Human skeletal muscle, Lane 3: Mouse heart, Lane 4: Rat heart.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunocytochemical analysis of MYL2 in A549 cells using a MYL2 Polyclonal antibody (Product # PA5-86045) as seen in green. The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunocytochemical analysis of MYL2 in C2C12 cells using a MYL2 Polyclonal antibody (Product # PA5-86045) as seen in green. The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemical analysis of MYL2 of paraffin-embedded Mouse heart tissue using a MYL2 Polyclonal antibody (Product #PA5-86045). Counter stained with hematoxylin.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemical analysis of MYL2 of paraffin-embedded rat heart tissue using a MYL2 Polyclonal antibody (Product #PA5-86045). Counter stained with hematoxylin.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Flow Cytometric analysis of MYL2 in Hela cells using a MYL2 Polyclonal Antibody (Product # PA5-86045) at a dilution of 1:100, as seen in blue compared with an unlabelled control (cells without incubation with primary antibody; red). Alexa Fluor 488-conjugated Goat anti rabbit IgG was used as the secondary antibody.