PA5-104265

antibody from Invitrogen Antibodies

Targeting: MYL2 CMH10

Western blot (Supportive data in Antibodypedia)

Immunocytochemistry (Supportive data in Antibodypedia)

Immunohistochemistry (Data presented on provider website)

Antibody Data

Product number

PA5-104265

Provider

Invitrogen Antibodies

Product name

Phospho-MYL2 (Ser15) Polyclonal Antibody

Antibody type

Polyclonal

Antigen

Synthetic peptide

Description

Antibody detects endogenous levels of MLC2 only when phosphorylated at Ser15.

Reactivity

Human, Mouse, Rat

Host

Rabbit

Isotype

IgG

Vial size

100 µL

Concentration

1 mg/mL

Storage

-20°C

References

Submitted references

Integrated multi-omics analysis of adverse cardiac remodeling and metabolic inflexibility upon ErbB2 and ERRα deficiency.

Dufour CR, Xia H, B'chir W, Perry MC, Kuzmanov U, Gainullina A, Dejgaard K, Scholtes C, Ouellet C, Zuo D, Sanguin-Gendreau V, Guluzian C, Smith HW, Muller WJ, Audet-Walsh E, Sergushichev AA, Emili A, Giguère V
Communications biology 2022 Sep 12;5(1):955

Western blot

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Western blot analysis of Phospho-MYL2 (Ser15) in rat muscle lysate. Samples were incubated with Phospho-MYL2 (Ser15) polyclonal antibody (Product # PA5-104265).

Immunocytochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunofluorescent analysis of Phospho-MYL2 (Ser15) in HepG2 during mitosis. Samples were fixed with paraformaldehyde, permeabilized with 0.1% Triton X-100, blocked with 10% serum (45 min at 25°C), incubated with mouse anti-beta tubulin and Phospho-MYL2 (Ser15) polyclonal antibody (Product # PA5-104265) using a dilution of 1:200 (1 hr, 37°C), and followed by goat anti-rabbit IgG Alexa Fluor 594 (red) and goat anti-mouse IgG Alexa Fluor 488 (green).

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunofluorescent analysis of Phospho-MYL2 (Ser15) in HepG2 during mitosis. Samples were fixed with paraformaldehyde, permeabilized with 0.1% Triton X-100, blocked with 10% serum (45 min at 25°C), incubated with mouse anti-beta tubulin and Phospho-MYL2 (Ser15) polyclonal antibody (Product # PA5-104265) using a dilution of 1:200 (1 hr, 37°C), and followed by goat anti-rabbit IgG Alexa Fluor 594 (red) and goat anti-mouse IgG Alexa Fluor 488 (green).

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunofluorescent analysis of Phospho-MYL2 (Ser15) in HepG2 during mitosis. Samples were fixed with paraformaldehyde, permeabilized with 0.1% Triton X-100, blocked with 10% serum (45 min at 25°C), incubated with mouse anti-beta tubulin and Phospho-MYL2 (Ser15) polyclonal antibody (Product # PA5-104265) using a dilution of 1:200 (1 hr, 37°C), and followed by goat anti-rabbit IgG Alexa Fluor 594 (red) and goat anti-mouse IgG Alexa Fluor 488 (green).