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Western blotAntibody data
- Antibody Data
- Antigen structure
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- Validations
- Western blot [1]
- Immunohistochemistry [3]
- Flow cytometry [1]
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- Product number
- F54326 - Provider product page
- Provider
- NSJ Bioreagents
- Product name
- Myosin Light Chain 2 Antibody / MLC2 / MYL2
- Antibody type
- Polyclonal
- Description
- This highly specific Myosin Light Chain 2 antibody is suitable for use in Western blot/Flow cytometry/Immunohistochemistry applications with human, mouse and rat samples.
- Reactivity
- Human, Mouse, Rat
- Host
- Rabbit
- Conjugate
- Unconjugated
- Vial size
- 0.05 ml, 0.2 ml
- Concentration
- In 1X PBS, pH 7.4, with 0.09% sodium azide
- Storage
- Aliquot the Myosin Light Chain 2 antibody and store frozen at -20oC or colder. Avoid repeated freeze-thaw cycles.
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Supportive validation
- Submitted by
- NSJ Bioreagents (provider)
- Main image
- Experimental details
- Western blot testing of 1) human heart, 2) human skeletal muscle, 3) mouse heart and 4) rat heart lysate with Myosin Light Chain 2 antibody. Predicted molecular weight ~19 kDa.
Supportive validation
- Submitted by
- NSJ Bioreagents (provider)
- Main image
- Experimental details
- IHC testing of FFPE human skeletal muscle tissue with Myosin Light Chain 2 antibody. HIER: steam section in pH6 citrate buffer for 20 min and allow to cool prior to staining.
- Submitted by
- NSJ Bioreagents (provider)
- Main image
- Experimental details
- IHC testing of FFPE human heart tissue with Myosin Light Chain 2 antibody. HIER: steam section in pH6 citrate buffer for 20 min and allow to cool prior to staining.
- Submitted by
- NSJ Bioreagents (provider)
- Main image
- Experimental details
- IHC testing of FFPE human myocardium tissue with Myosin Light Chain 2 antibody. HIER: steam section in pH6 citrate buffer for 20 min and allow to cool prior to staining.
Supportive validation
- Submitted by
- NSJ Bioreagents (provider)
- Main image
- Experimental details
- Flow cytometry testing of fixed and permeabilized human U-2 OS cells with Myosin Light Chain 2 antibody; Blue=isotype control, Green= Myosin Light Chain 2 antibody.
