21935-1-AP

antibody from Invitrogen Antibodies

Targeting: POLD3 KIAA0039, P66, P68, PPP1R128

Western blot (Supportive data in Antibodypedia)

Immunocytochemistry (Supportive data in Antibodypedia)

Immunoprecipitation (Data presented on provider website)

Immunohistochemistry (Supportive data in Antibodypedia)

Other assay (Supportive data in Antibodypedia)

Antibody Data

Product number

21935-1-AP

Provider

Invitrogen Antibodies

Product name

POLD3 Polyclonal Antibody

Antibody type

Polyclonal

Antigen

Other

Description

Immunogen sequence: MLKDSGPLF NTDYDILKSN LQNCSKFSAI QCAAAVPRAP AESSSSSKKF EQSHLHMSSE TQANNELTTN GHGPPASKQV SQQPKGIMGM FASKAAAKTQ ETNKETKTEA KEVTNASAAG NKAPGKGNMM SNFFGKAAMN KFKVNLDSEQ AVKEEKIVEQ PTVSVTEPKL ATPAGLKKSS KKAEPVKVLQ KEKKRGKRVA LSDDETKETE NMRKKRRRIK LPESDSSEDE VFPDSPGAYE AESPSPPPPP SPPLEPVPKT EPEPPSVKSS SGENKRKRKR VLKSKTYLDG EGCIVTEKVY ESESCTDSEE ELNMKTSSVH RPPAMTVKKE PREERKGPKK GTAALGKANR QVSITGFFQR K (107-466 aa encoded by BC108908)

Reactivity

Human, Mouse, Rat

Host

Rabbit

Isotype

IgG

Vial size

150 µL

Concentration

0.37 mg/mL

Storage

-20°C

Western blot

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

MCF-7 cells were subjected to SDS PAGE followed by western blot with 21935-1-AP ( POLD3 Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Mouse spleen tissue were subjected to SDS PAGE followed by western blot with 21935-1-AP (POLD3 Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

Immunocytochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunofluorescent analysis of MCF-7 cells using 21935-1-AP (POLD3 antibody) at dilution of 1:25 and Rhodamine-Goat anti-Rabbit IGG.

Immunohistochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemistry of paraffin-embedded human breast cancer tissue slide using 21935-1-AP (POLD3 Antibody) at dilution of 1:50 (under 10x lens).

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemistry of paraffin-embedded human breast cancer tissue slide using 21935-1-AP (POLD3 Antibody) at dilution of 1:50 (under 40x lens).

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemistry of paraffin-embedded human cervical cancer tissue slide using 21935-1-AP ( POLD3 Antibody) at dilution of 1:50 (under 10x lens).

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemistry of paraffin-embedded human cervical cancer tissue slide using 21935-1-AP ( POLD3 Antibody) at dilution of 1:50 (under 40x lens).

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using (Product # 21935-1-AP) (POLD3 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

Other assay

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

IP result of anti-POLD3 (IP:21935-1-AP, 4ug; Detection:21935-1-AP 1:300) with MCF-7 cells lysate 2560ug.