A301-243A

antibody from Invitrogen Antibodies

Targeting: POLD3 KIAA0039, P66, P68, PPP1R128

Western blot (Recommended by provider)

Immunoprecipitation (Data presented on provider website)

Immunohistochemistry (Supportive data in Antibodypedia)

Other assay (Supportive data in Antibodypedia)

Antibody Data

Product number

A301-243A

Provider

Invitrogen Antibodies

Product name

PolD3/p66 Polyclonal Antibody

Antibody type

Polyclonal

Antigen

Other

Reactivity

Human

Host

Rabbit

Isotype

IgG

Vial size

100 µL

Concentration

1 mg/mL

Storage

4° C

Immunohistochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Detection of human PolD3/p66 by immunohistochemistry. Sample: FFPE section of human small cell lung cancer. Antibody: Affinity purified rabbit anti- PolD3/p66 (Cat. No. A301-243A Lot1) used at a dilution of 1:1,000 (1µg/ml). Detection: DAB.

Other assay

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Detection of human PolD3/p66 by western blot of immunoprecipitates. Samples: Whole cell lysate (1 mg for IP, 20% of IP loaded) from HeLa cells. Antibodies: Affinity purified rabbit anti-PolD3/p66 antibody A301-243A used for IP at 3 µg/mg lysate. PolD3/p66 was also immunoprecipitated by rabbit anti-PolD3/p66 antibody BL5048, which recognizes an upstream epitope. For blotting immunoprecipitated PolD3/p66, A301-244A was used at 1 µg/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.