14886-1-AP

antibody from Proteintech Group

Targeting: GOT1 AST1

Provider product page for 14886-1-AP

Western blot

ELISA

Immunocytochemistry

Immunoprecipitation

Antibody data

Antibody Data
Antigen structure
References [36]
Comments [0]
Validations
Western blot [1]

Submit

Product number: 14886-1-AP - Provider product page
Provider: Proteintech Group
Proper citation: Proteintech Cat#14886-1-AP, RRID:AB_2113630
Product name: GOT1 antibody
Antibody type: Polyclonal
Description: KD/KO validated GOT1 antibody (Cat. #14886-1-AP) is a rabbit polyclonal antibody that shows reactivity with human, mouse, rat and has been validated for the following applications: IF, IP, WB,ELISA.
Reactivity: Human, Mouse, Rat
Host: Rabbit
Conjugate: Unconjugated
Isotype: IgG
Vial size: 20ul, 150ul

GOT1 protein structure - 14886-1-AP shown in red.

Submitted references Testosterone regulates thymic remodeling by altering metabolic reprogramming in male rats.

Li D, Yao H, Ren Y, Shang J, Han X, Cao X, Song T, Zeng X
General and comparative endocrinology 2024 Mar 1;348:114448

Effects of gas signaling molecule SO(2) in cardiac functions of hyperthyroid rats.

Yang Q, Yang T, Liu X, Liu S, Liu W, Nie L, Chu C, Yang J
The Korean journal of physiology & pharmacology : official journal of the Korean Physiological Society and the Korean Society of Pharmacology 2024 Mar 1;28(2):129-143

PoRVA G9P[23] and G5P[7] infections differentially promote PEDV replication by reprogramming glutamine metabolism.

Liu H, Tian H, Hao P, Du H, Wang K, Qiu Y, Yin X, Wu N, Du Q, Tong D, Huang Y
PLoS pathogens 2024 Jun;20(6):e1012305

Metabolic priming by multiple enzyme systems supports glycolysis, HIF1α stabilisation, and human cancer cell survival in early hypoxia.

Grimm F, Asuaje A, Jain A, Silva Dos Santos M, Kleinjung J, Nunes PM, Gehrig S, Fets L, Darici S, MacRae JI, Anastasiou D
The EMBO journal 2024 Apr;43(8):1545-1569

HIF1α-dependent uncoupling of glycolysis suppresses tumor cell proliferation.

Urrutia AA, Mesa-Ciller C, Guajardo-Grence A, Alkan HF, Soro-Arnáiz I, Vandekeere A, Ferreira Campos AM, Igelmann S, Fernández-Arroyo L, Rinaldi G, Lorendeau D, De Bock K, Fendt SM, Aragonés J
Cell reports 2024 Apr 23;43(4):114103

Role of asparagine biosynthesis pathway in Siniperca chuatsi rhabdovirus proliferation.

Li F, Fu X, Luo X, Lin Q, Liang H, Niu Y, Liu L, Li N
Frontiers in microbiology 2023;14:1165491

Label-free quantitative proteomic analyses of mouse astrocytes provides insight into the host response mechanism at different developmental stages of Toxoplasma gondii.

Xie H, Sun H, Dong H, Dai L, Xu H, Zhang L, Wang Q, Zhang J, Zhao G, Xu C, Yin K
PLoS neglected tropical diseases 2023 Sep;17(9):e0011102

ASS1-Mediated Reductive Carboxylation of Cytosolic Glutamine Confers Ferroptosis Resistance in Cancer Cells.

Hu Q, Dai J, Zhang Z, Yu H, Zhang J, Zhu X, Qin Y, Zhang L, Zhang P
Cancer research 2023 May 15;83(10):1646-1665

De novo pyrimidine biosynthetic complexes support cancer cell proliferation and ferroptosis defence.

Yang C, Zhao Y, Wang L, Guo Z, Ma L, Yang R, Wu Y, Li X, Niu J, Chu Q, Fu Y, Li B
Nature cell biology 2023 Jun;25(6):836-847

A coordinated multiorgan metabolic response contributes to human mitochondrial myopathy.

Southwell N, Primiano G, Nadkarni V, Attarwala N, Beattie E, Miller D, Alam S, Liparulo I, Shurubor YI, Valentino ML, Carelli V, Servidei S, Gross SS, Manfredi G, Chen Q, D'Aurelio M
EMBO molecular medicine 2023 Jul 10;15(7):e16951

Metabolic clearance of oxaloacetate and mitochondrial complex II respiration: Divergent control in skeletal muscle and brown adipose tissue.

Yu L, Fink BD, Som R, Rauckhorst AJ, Taylor EB, Sivitz WI
Biochimica et biophysica acta. Bioenergetics 2023 Jan 1;1864(1):148930

Cryptotanshinone suppresses tumorigenesis by inhibiting lipogenesis and promoting reactive oxygen species production in KRAS‑activated pancreatic cancer cells.

Terado T, Kim CJ, Ushio A, Minami K, Tambe Y, Kageyama S, Kawauchi A, Tsunoda T, Shirasawa S, Tanaka H, Inoue H
International journal of oncology 2022 Sep;61(3)

Gaseous signal molecule SO(2) regulates autophagy through PI3K/AKT pathway inhibits cardiomyocyte apoptosis and improves myocardial fibrosis in rats with type II diabetes.

Zhao J, Wu Q, Yang T, Nie L, Liu S, Zhou J, Chen J, Jiang Z, Xiao T, Yang J, Chu C
The Korean journal of physiology & pharmacology : official journal of the Korean Physiological Society and the Korean Society of Pharmacology 2022 Nov 1;26(6):541-556

Systematic discovery of mutation-directed neo-protein-protein interactions in cancer.

Mo X, Niu Q, Ivanov AA, Tsang YH, Tang C, Shu C, Li Q, Qian K, Wahafu A, Doyle SP, Cicka D, Yang X, Fan D, Reyna MA, Cooper LAD, Moreno CS, Zhou W, Owonikoko TK, Lonial S, Khuri FR, Du Y, Ramalingam SS, Mills GB, Fu H
Cell 2022 May 26;185(11):1974-1985.e12

Loss of Heterozygosity for Kras(G12D) Promotes Malignant Phenotype of Pancreatic Ductal Adenocarcinoma by Activating HIF-2α-c-Myc-Regulated Glutamine Metabolism.

Ma Y, Ling S, Li Y, Hu M, Kong B, Huang P, Liu H
International journal of molecular sciences 2022 Jun 15;23(12)

Integrative proteomics and metabolomics approach to elucidate metabolic dysfunction induced by silica nanoparticles in hepatocytes.

Zhu Y, Zhang Y, Li Y, Guo C, Fan Z, Li Y, Yang M, Zhou X, Sun Z, Wang J
Journal of hazardous materials 2022 Jul 15;434:128820

A redox cycle with complex II prioritizes sulfide quinone oxidoreductase-dependent H(2)S oxidation.

Kumar R, Landry AP, Guha A, Vitvitsky V, Lee HJ, Seike K, Reddy P, Lyssiotis CA, Banerjee R
The Journal of biological chemistry 2022 Jan;298(1):101435

Glutamine synthetase limits β-catenin-mutated liver cancer growth by maintaining nitrogen homeostasis and suppressing mTORC1.

Dai W, Shen J, Yan J, Bott AJ, Maimouni S, Daguplo HQ, Wang Y, Khayati K, Guo JY, Zhang L, Wang Y, Valvezan A, Ding WX, Chen X, Su X, Gao S, Zong WX
The Journal of clinical investigation 2022 Dec 15;132(24)

Medioresinol as a novel PGC-1α activator prevents pyroptosis of endothelial cells in ischemic stroke through PPARα-GOT1 axis.

Wang Y, Guan X, Gao CL, Ruan W, Zhao S, Kai G, Li F, Pang T
Pharmacological research 2021 Jul;169:105640

The mitochondrial NADH pool is involved in hydrogen sulfide signaling and stimulation of aerobic glycolysis.

Vitvitsky V, Kumar R, Libiad M, Maebius A, Landry AP, Banerjee R
The Journal of biological chemistry 2021 Jan-Jun;296:100736

Contribution of Connexin Hemichannels to the Pathogenesis of Acute Lung Injury.

Wang S, Sun Y, Bai Y, Zhou N, Chen N, Miller EJ, Zhang Y, Li W
Mediators of inflammation 2020;2020:8094347

Inhibiting both proline biosynthesis and lipogenesis synergistically suppresses tumor growth.

Liu M, Wang Y, Yang C, Ruan Y, Bai C, Chu Q, Cui Y, Chen C, Ying G, Li B
The Journal of experimental medicine 2020 Mar 2;217(3)

Ischemic Neuroprotectant PKCε Restores Mitochondrial Glutamate Oxaloacetate Transaminase in the Neuronal NADH Shuttle after Ischemic Injury.

Xu J, Khoury N, Jackson CW, Escobar I, Stegelmann SD, Dave KR, Perez-Pinzon MA
Translational stroke research 2020 Jun;11(3):418-432

Glutamine Anabolism Plays a Critical Role in Pancreatic Cancer by Coupling Carbon and Nitrogen Metabolism.

Bott AJ, Shen J, Tonelli C, Zhan L, Sivaram N, Jiang YP, Yu X, Bhatt V, Chiles E, Zhong H, Maimouni S, Dai W, Velasquez S, Pan JA, Muthalagu N, Morton J, Anthony TG, Feng H, Lamers WH, Murphy DJ, Guo JY, Jin J, Crawford HC, Zhang L, White E, Lin RZ, Su X, Tuveson DA, Zong WX
Cell reports 2019 Oct 29;29(5):1287-1298.e6

Glutamine Metabolism Is Required for Collagen Protein Synthesis in Lung Fibroblasts.

Hamanaka RB, O'Leary EM, Witt LJ, Tian Y, Gökalp GA, Meliton AY, Dulin NO, Mutlu GM
American journal of respiratory cell and molecular biology 2019 Nov;61(5):597-606

Coordinative metabolism of glutamine carbon and nitrogen in proliferating cancer cells under hypoxia.

Wang Y, Bai C, Ruan Y, Liu M, Chu Q, Qiu L, Yang C, Li B
Nature communications 2019 Jan 14;10(1):201

HIF1α Suppresses Tumor Cell Proliferation through Inhibition of Aspartate Biosynthesis.

Meléndez-Rodríguez F, Urrutia AA, Lorendeau D, Rinaldi G, Roche O, Böğürcü-Seidel N, Ortega Muelas M, Mesa-Ciller C, Turiel G, Bouthelier A, Hernansanz-Agustín P, Elorza A, Escasany E, Li QOY, Torres-Capelli M, Tello D, Fuertes E, Fraga E, Martínez-Ruiz A, Pérez B, Giménez-Bachs JM, Salinas-Sánchez AS, Acker T, Sánchez Prieto R, Fendt SM, De Bock K, Aragonés J
Cell reports 2019 Feb 26;26(9):2257-2265.e4

Quantitative In Vivo Proteomics of Metformin Response in Liver Reveals AMPK-Dependent and -Independent Signaling Networks.

Stein BD, Calzolari D, Hellberg K, Hu YS, He L, Hung CM, Toyama EQ, Ross DS, Lillemeier BF, Cantley LC, Yates JR 3rd, Shaw RJ
Cell reports 2019 Dec 3;29(10):3331-3348.e7

Rewiring of Glutamine Metabolism Is a Bioenergetic Adaptation of Human Cells with Mitochondrial DNA Mutations.

Chen Q, Kirk K, Shurubor YI, Zhao D, Arreguin AJ, Shahi I, Valsecchi F, Primiano G, Calder EL, Carelli V, Denton TT, Beal MF, Gross SS, Manfredi G, D'Aurelio M
Cell metabolism 2018 May 1;27(5):1007-1025.e5

Sirtuin5 contributes to colorectal carcinogenesis by enhancing glutaminolysis in a deglutarylation-dependent manner.

Wang YQ, Wang HL, Xu J, Tan J, Fu LN, Wang JL, Zou TH, Sun DF, Gao QY, Chen YX, Fang JY
Nature communications 2018 Feb 7;9(1):545

Glutaminase GLS1 senses glutamine availability in a non-enzymatic manner triggering mitochondrial fusion.

Cai WF, Zhang C, Wu YQ, Zhuang G, Ye Z, Zhang CS, Lin SC
Cell research 2018 Aug;28(8):865-867

Cooperative STAT/NF-κB signaling regulates lymphoma metabolic reprogramming and aberrant GOT2 expression.

Feist M, Schwarzfischer P, Heinrich P, Sun X, Kemper J, von Bonin F, Perez-Rubio P, Taruttis F, Rehberg T, Dettmer K, Gronwald W, Reinders J, Engelmann JC, Dudek J, Klapper W, Trümper L, Spang R, Oefner PJ, Kube D
Nature communications 2018 Apr 17;9(1):1514

Gaseous signalling molecule SO2 via Hippo‑MST pathway to improve myocardial fibrosis of diabetic rats.

Liu M, Liu S, Tan W, Tang F, Long J, Li Z, Liang B, Chu C, Yang J
Molecular medicine reports 2017 Dec;16(6):8953-8963

Identification of Organ-Enriched Protein Biomarkers of Acute Liver Injury by Targeted Quantitative Proteomics of Blood in Acetaminophen- and Carbon-Tetrachloride-Treated Mouse Models and Acetaminophen Overdose Patients.

Qin S, Zhou Y, Gray L, Kusebauch U, McEvoy L, Antoine DJ, Hampson L, Park KB, Campbell D, Caballero J, Glusman G, Yan X, Kim TK, Yuan Y, Wang K, Rowen L, Moritz RL, Omenn GS, Pirmohamed M, Hood L
Journal of proteome research 2016 Oct 7;15(10):3724-3740

cMyc-mediated activation of serine biosynthesis pathway is critical for cancer progression under nutrient deprivation conditions.

Sun L, Song L, Wan Q, Wu G, Li X, Wang Y, Wang J, Liu Z, Zhong X, He X, Shen S, Pan X, Li A, Wang Y, Gao P, Tang H, Zhang H
Cell research 2015 Apr;25(4):429-44

Enhanced synthesis and diminished degradation of hydrogen sulfide in experimental colitis: a site-specific, pro-resolution mechanism.

Flannigan KL, Ferraz JG, Wang R, Wallace JL
PloS one 2013;8(8):e71962

No comments: Submit comment

Supportive validation

Submitted by: Proteintech Group (provider)
Main image
Experimental details: The GOT1 antibody from Proteintech is a rabbit polyclonal antibody to a recombinant protein of human GOT1. This antibody recognizes human,mouse,rat antigen. The GOT1 antibody has been validated for the following applications: ELISA, WB analysis.
Appendix: 14886-1-AP.pdf

: Show more