14886-1-AP

antibody from Proteintech Group

Targeting: GOT1 AST1

Western blot (Supportive data in Antibodypedia)

ELISA (Data presented on provider website)

Immunocytochemistry (Data presented on provider website)

Immunoprecipitation (Data presented on provider website)

Antibody Data

Product number

14886-1-AP

Provider

Proteintech Group

Proper citation

Proteintech Cat#14886-1-AP, RRID:AB_2113630

Product name

GOT1 antibody

Antibody type

Polyclonal

Description

KD/KO validated GOT1 antibody (Cat. #14886-1-AP) is a rabbit polyclonal antibody that shows reactivity with human, mouse, rat and has been validated for the following applications: IF, IP, WB,ELISA.

Reactivity

Human, Mouse, Rat

Host

Rabbit

Conjugate

Unconjugated

Isotype

IgG

Vial size

20ul, 150ul

References

Submitted references

Aspartate availability drives differential engagement of the malate-aspartate shuttle.

Brunner JS, Bridgeman AE, Jackson BT, Chakraborty S, Fagoaga-Eugui M, Paras KI, Xie A, Arnold PK, Losner J, Finley LWS
Molecular cell 2026 Mar 5;86(5):954-967.e7

---

Tumor metabolic adaptation induced by L-asparaginase reveals a vulnerability to PARP1/2 inhibitor in B-cell lymphomas.

Aussel A, Nemazanyy I, Vandenberghe A, Meola P, Dorat N, Paul R, Habbouche L, Exposito L, Nottet N, Petrilli V, Chiche E, Mounier N, Marchetti S, Chaveroux C, Ricci JE, Chiche J
Nature communications 2026 Feb 27;17(1)

---

Circadian tryptophan metabolism contributes to systemic aryl hydrocarbon receptor activity.

Morgan EW, Murray IA, Coslo DM, Patterson AD, Perdew GH
iScience 2026 Feb 20;29(2):114680

---

Oncolytic virus hijacks GOT1 and pyrimidinosomes to fuel pyrimidine synthesis for replication in tumor cells.

Tang N, Gong Y, Zhao C, Niu Y, Tan L, Song C, Qiu X, Liao Y, Yu S, Ding C, Lin S, Sun Y
Tumour virus research 2026 Apr 9;21:200342

---

Scoparone enhances the stability of TAT protein by inhibiting the Ubiquitin-Proteasome pathway, regulates mitochondrial damage against chronic alcoholic liver disease.

Wang C, Sun Y, Yang L, Ren J, Fang H, Sun Y, Yan G, Kong L, Han Y, Wang X, Sun H
Journal of ethnopharmacology 2026 Apr 25;368:121746

---

Oxaloacetate sensing promotes innate immune antiviral defence against influenza virus infection.

Jin S, He X, Wang Z, Zhou T, Wang J, Pan G, Zhang Y, Ma L, Yang S, Wang L, Wu Y, Zou Y, Qi N, Cui J
Nature microbiology 2025 Oct;10(10):2521-2536

---

Pyridoxal phosphate inhibits alpha-synuclein-induced ferroptosis by activating GOT1 to enhance the methionine salvage pathway in Parkinson's disease.

Min X, Du Z, Wei J, Yuan Z, She Y, Jin X, Su Z, Mao H, Cai J, Zhu Z, He X
Experimental neurology 2025 Nov;393:115411

---

Subacute exposure to thallium disrupts renal energy metabolism by impairing the function of subcellular organelles in aged mice of both sexes.

Yao H, Li L, Dai Q, Li D
Food and chemical toxicology : an international journal published for the British Industrial Biological Research Association 2025 Nov;205:115700

---

African swine fever virus hijacks host pyrimidine metabolism to promote viral replication.

Song Z, Chen Y, Guo H, Zhang G, Gong L, Zheng Z
Journal of virology 2025 Nov 25;99(11):e0098525

---

SIRT5-mediated desuccinylation of the porcine deltacoronavirus M protein drives pexophagy to enhance viral proliferation.

Li Z, Tang W, Lai Y, Chen C, Fang P, Zhou Y, Fang L, Xiao S
PLoS pathogens 2025 May;21(5):e1013163

---

Cleavage of CAD by caspase-3 determines the cancer cell fate during chemotherapy.

Ma J, Zhao J, Zhang C, Tan J, Cheng A, Niu Z, Lin Z, Pan G, Chen C, Ding Y, Zhong M, Zhuang Y, Xiong Y, Zhou H, Zhou S, Xu M, Ye W, Li F, Song Y, Wang Z, Hong X
Nature communications 2025 May 30;16(1):5006

---

ANXA1 promotes intrahepatic cholangiocarcinoma proliferation and growth by regulating glutamine metabolism through GOT1 stabilization.

Gong Y, Chen L, Wang H, Zheng D, Li F, Wu C, Li Y, Deng Y, He Z, Yu C
Journal of experimental & clinical cancer research : CR 2025 May 19;44(1):151

---

Mitochondrial glutamic-oxaloacetic transaminase (GOT2) in the growth of C2C12 myoblasts.

Som R, Fink BD, Rauckhorst AJ, Taylor EB, Sivitz WI
Journal of bioenergetics and biomembranes 2025 Jun;57(2-3):85-95

---

Rhein Inhibits Microglia-Mediated Neuroinflammation and Neuronal Damage of Alzheimer's Disease via Regulating the Glutamine-Aspartate-Arginine-NO Metabolic Pathway.

Chi B, Zhang Z, Zhang Z, Zhang H, Tian M, Wang Y, Gao X
International journal of molecular sciences 2025 Jul 3;26(13)

---

Aspergillus fumigatus promotes tumor angiogenesis via SLC7A11 on myeloid-derived suppressor cells.

Qu W, Wang Z, Zhu T, Cui H, Bing Z, Shen S, Shen Y, Yu S, Zhuang H, Wang T
EMBO reports 2025 Dec;26(24):6266-6291

---

DGKζ in Glycerophospholipid Metabolism Regulates the DAG and PA Balance and Interacts With PTEN to Alleviate Brain Damage in Septic Mice With Hydrogen Inhalation: A Comparative Metabolomic and Phosphoproteomic Analysis.

Bai Y, Li Z, Yan D, Jiang Y, Dong B, Yu Y
Brain and behavior 2025 Aug;15(8):e70761

---

Asparagine Availability Is a Critical Limiting Factor for Infectious Spleen and Kidney Necrosis Virus Replication.

Ma B, Li F, Fu X, Luo X, Lin Q, Liang H, Niu Y, Li N
Viruses 2024 Sep 29;16(10)

---

Glutaminolysis provides nucleotides and amino acids to regulate osteoclast differentiation in mice.

Hu G, Yu Y, Ren Y, Tower RJ, Zhang GF, Karner CM
EMBO reports 2024 Oct;25(10):4515-4541

---

SIRT1 maintains bone homeostasis by regulating osteoblast glycolysis through GOT1.

Jin X, Sun X, Ma X, Qin Z, Gao X, Kang X, Li H, Sun H
Cellular and molecular life sciences : CMLS 2024 May 3;81(1):204

---

Testosterone regulates thymic remodeling by altering metabolic reprogramming in male rats.

Li D, Yao H, Ren Y, Shang J, Han X, Cao X, Song T, Zeng X
General and comparative endocrinology 2024 Mar 1;348:114448

---

Effects of gas signaling molecule SO(2) in cardiac functions of hyperthyroid rats.

Yang Q, Yang T, Liu X, Liu S, Liu W, Nie L, Chu C, Yang J
The Korean journal of physiology & pharmacology : official journal of the Korean Physiological Society and the Korean Society of Pharmacology 2024 Mar 1;28(2):129-143

---

PoRVA G9P[23] and G5P[7] infections differentially promote PEDV replication by reprogramming glutamine metabolism.

Liu H, Tian H, Hao P, Du H, Wang K, Qiu Y, Yin X, Wu N, Du Q, Tong D, Huang Y
PLoS pathogens 2024 Jun;20(6):e1012305

---

BET inhibition induces GDH1-dependent glutamine metabolic remodeling and vulnerability in liver cancer.

Mi W, You J, Li L, Zhu L, Xia X, Yang L, Li F, Xu Y, Bi J, Liu P, Chen L, Li F
Life metabolism 2024 Aug;3(4):loae016

---

Metabolic priming by multiple enzyme systems supports glycolysis, HIF1α stabilisation, and human cancer cell survival in early hypoxia.

Grimm F, Asuaje A, Jain A, Silva Dos Santos M, Kleinjung J, Nunes PM, Gehrig S, Fets L, Darici S, MacRae JI, Anastasiou D
The EMBO journal 2024 Apr;43(8):1545-1569

---

HIF1α-dependent uncoupling of glycolysis suppresses tumor cell proliferation.

Urrutia AA, Mesa-Ciller C, Guajardo-Grence A, Alkan HF, Soro-Arnáiz I, Vandekeere A, Ferreira Campos AM, Igelmann S, Fernández-Arroyo L, Rinaldi G, Lorendeau D, De Bock K, Fendt SM, Aragonés J
Cell reports 2024 Apr 23;43(4):114103

---

Role of asparagine biosynthesis pathway in Siniperca chuatsi rhabdovirus proliferation.

Li F, Fu X, Luo X, Lin Q, Liang H, Niu Y, Liu L, Li N
Frontiers in microbiology 2023;14:1165491

---

Label-free quantitative proteomic analyses of mouse astrocytes provides insight into the host response mechanism at different developmental stages of Toxoplasma gondii.

Xie H, Sun H, Dong H, Dai L, Xu H, Zhang L, Wang Q, Zhang J, Zhao G, Xu C, Yin K
PLoS neglected tropical diseases 2023 Sep;17(9):e0011102

---

ASS1-Mediated Reductive Carboxylation of Cytosolic Glutamine Confers Ferroptosis Resistance in Cancer Cells.

Hu Q, Dai J, Zhang Z, Yu H, Zhang J, Zhu X, Qin Y, Zhang L, Zhang P
Cancer research 2023 May 15;83(10):1646-1665

---

De novo pyrimidine biosynthetic complexes support cancer cell proliferation and ferroptosis defence.

Yang C, Zhao Y, Wang L, Guo Z, Ma L, Yang R, Wu Y, Li X, Niu J, Chu Q, Fu Y, Li B
Nature cell biology 2023 Jun;25(6):836-847

---

A coordinated multiorgan metabolic response contributes to human mitochondrial myopathy.

Southwell N, Primiano G, Nadkarni V, Attarwala N, Beattie E, Miller D, Alam S, Liparulo I, Shurubor YI, Valentino ML, Carelli V, Servidei S, Gross SS, Manfredi G, Chen Q, D'Aurelio M
EMBO molecular medicine 2023 Jul 10;15(7):e16951

---

Metabolic clearance of oxaloacetate and mitochondrial complex II respiration: Divergent control in skeletal muscle and brown adipose tissue.

Yu L, Fink BD, Som R, Rauckhorst AJ, Taylor EB, Sivitz WI
Biochimica et biophysica acta. Bioenergetics 2023 Jan 1;1864(1):148930

---

Cryptotanshinone suppresses tumorigenesis by inhibiting lipogenesis and promoting reactive oxygen species production in KRAS‑activated pancreatic cancer cells.

Terado T, Kim CJ, Ushio A, Minami K, Tambe Y, Kageyama S, Kawauchi A, Tsunoda T, Shirasawa S, Tanaka H, Inoue H
International journal of oncology 2022 Sep;61(3)

---

Gaseous signal molecule SO(2) regulates autophagy through PI3K/AKT pathway inhibits cardiomyocyte apoptosis and improves myocardial fibrosis in rats with type II diabetes.

Zhao J, Wu Q, Yang T, Nie L, Liu S, Zhou J, Chen J, Jiang Z, Xiao T, Yang J, Chu C
The Korean journal of physiology & pharmacology : official journal of the Korean Physiological Society and the Korean Society of Pharmacology 2022 Nov 1;26(6):541-556

---

Systematic discovery of mutation-directed neo-protein-protein interactions in cancer.

Mo X, Niu Q, Ivanov AA, Tsang YH, Tang C, Shu C, Li Q, Qian K, Wahafu A, Doyle SP, Cicka D, Yang X, Fan D, Reyna MA, Cooper LAD, Moreno CS, Zhou W, Owonikoko TK, Lonial S, Khuri FR, Du Y, Ramalingam SS, Mills GB, Fu H
Cell 2022 May 26;185(11):1974-1985.e12

---

Loss of Heterozygosity for Kras(G12D) Promotes Malignant Phenotype of Pancreatic Ductal Adenocarcinoma by Activating HIF-2α-c-Myc-Regulated Glutamine Metabolism.

Ma Y, Ling S, Li Y, Hu M, Kong B, Huang P, Liu H
International journal of molecular sciences 2022 Jun 15;23(12)

---

Integrative proteomics and metabolomics approach to elucidate metabolic dysfunction induced by silica nanoparticles in hepatocytes.

Zhu Y, Zhang Y, Li Y, Guo C, Fan Z, Li Y, Yang M, Zhou X, Sun Z, Wang J
Journal of hazardous materials 2022 Jul 15;434:128820

---

A redox cycle with complex II prioritizes sulfide quinone oxidoreductase-dependent H(2)S oxidation.

Kumar R, Landry AP, Guha A, Vitvitsky V, Lee HJ, Seike K, Reddy P, Lyssiotis CA, Banerjee R
The Journal of biological chemistry 2022 Jan;298(1):101435

---

Glutamine synthetase limits β-catenin-mutated liver cancer growth by maintaining nitrogen homeostasis and suppressing mTORC1.

Dai W, Shen J, Yan J, Bott AJ, Maimouni S, Daguplo HQ, Wang Y, Khayati K, Guo JY, Zhang L, Wang Y, Valvezan A, Ding WX, Chen X, Su X, Gao S, Zong WX
The Journal of clinical investigation 2022 Dec 15;132(24)

---

Medioresinol as a novel PGC-1α activator prevents pyroptosis of endothelial cells in ischemic stroke through PPARα-GOT1 axis.

Wang Y, Guan X, Gao CL, Ruan W, Zhao S, Kai G, Li F, Pang T
Pharmacological research 2021 Jul;169:105640

---

The mitochondrial NADH pool is involved in hydrogen sulfide signaling and stimulation of aerobic glycolysis.

Vitvitsky V, Kumar R, Libiad M, Maebius A, Landry AP, Banerjee R
The Journal of biological chemistry 2021 Jan-Jun;296:100736

---

Contribution of Connexin Hemichannels to the Pathogenesis of Acute Lung Injury.

Wang S, Sun Y, Bai Y, Zhou N, Chen N, Miller EJ, Zhang Y, Li W
Mediators of inflammation 2020;2020:8094347

---

Inhibiting both proline biosynthesis and lipogenesis synergistically suppresses tumor growth.

Liu M, Wang Y, Yang C, Ruan Y, Bai C, Chu Q, Cui Y, Chen C, Ying G, Li B
The Journal of experimental medicine 2020 Mar 2;217(3)

---

Ischemic Neuroprotectant PKCε Restores Mitochondrial Glutamate Oxaloacetate Transaminase in the Neuronal NADH Shuttle after Ischemic Injury.

Xu J, Khoury N, Jackson CW, Escobar I, Stegelmann SD, Dave KR, Perez-Pinzon MA
Translational stroke research 2020 Jun;11(3):418-432

---

Glutamine Anabolism Plays a Critical Role in Pancreatic Cancer by Coupling Carbon and Nitrogen Metabolism.

Bott AJ, Shen J, Tonelli C, Zhan L, Sivaram N, Jiang YP, Yu X, Bhatt V, Chiles E, Zhong H, Maimouni S, Dai W, Velasquez S, Pan JA, Muthalagu N, Morton J, Anthony TG, Feng H, Lamers WH, Murphy DJ, Guo JY, Jin J, Crawford HC, Zhang L, White E, Lin RZ, Su X, Tuveson DA, Zong WX
Cell reports 2019 Oct 29;29(5):1287-1298.e6

---

Glutamine Metabolism Is Required for Collagen Protein Synthesis in Lung Fibroblasts.

Hamanaka RB, O'Leary EM, Witt LJ, Tian Y, Gökalp GA, Meliton AY, Dulin NO, Mutlu GM
American journal of respiratory cell and molecular biology 2019 Nov;61(5):597-606

---

Coordinative metabolism of glutamine carbon and nitrogen in proliferating cancer cells under hypoxia.

Wang Y, Bai C, Ruan Y, Liu M, Chu Q, Qiu L, Yang C, Li B
Nature communications 2019 Jan 14;10(1):201

---

HIF1α Suppresses Tumor Cell Proliferation through Inhibition of Aspartate Biosynthesis.

Meléndez-Rodríguez F, Urrutia AA, Lorendeau D, Rinaldi G, Roche O, Böğürcü-Seidel N, Ortega Muelas M, Mesa-Ciller C, Turiel G, Bouthelier A, Hernansanz-Agustín P, Elorza A, Escasany E, Li QOY, Torres-Capelli M, Tello D, Fuertes E, Fraga E, Martínez-Ruiz A, Pérez B, Giménez-Bachs JM, Salinas-Sánchez AS, Acker T, Sánchez Prieto R, Fendt SM, De Bock K, Aragonés J
Cell reports 2019 Feb 26;26(9):2257-2265.e4

---

Quantitative In Vivo Proteomics of Metformin Response in Liver Reveals AMPK-Dependent and -Independent Signaling Networks.

Stein BD, Calzolari D, Hellberg K, Hu YS, He L, Hung CM, Toyama EQ, Ross DS, Lillemeier BF, Cantley LC, Yates JR 3rd, Shaw RJ
Cell reports 2019 Dec 3;29(10):3331-3348.e7

---

Rewiring of Glutamine Metabolism Is a Bioenergetic Adaptation of Human Cells with Mitochondrial DNA Mutations.

Chen Q, Kirk K, Shurubor YI, Zhao D, Arreguin AJ, Shahi I, Valsecchi F, Primiano G, Calder EL, Carelli V, Denton TT, Beal MF, Gross SS, Manfredi G, D'Aurelio M
Cell metabolism 2018 May 1;27(5):1007-1025.e5

---

Sirtuin5 contributes to colorectal carcinogenesis by enhancing glutaminolysis in a deglutarylation-dependent manner.

Wang YQ, Wang HL, Xu J, Tan J, Fu LN, Wang JL, Zou TH, Sun DF, Gao QY, Chen YX, Fang JY
Nature communications 2018 Feb 7;9(1):545

---

Glutaminase GLS1 senses glutamine availability in a non-enzymatic manner triggering mitochondrial fusion.

Cai WF, Zhang C, Wu YQ, Zhuang G, Ye Z, Zhang CS, Lin SC
Cell research 2018 Aug;28(8):865-867

---

Cooperative STAT/NF-κB signaling regulates lymphoma metabolic reprogramming and aberrant GOT2 expression.

Feist M, Schwarzfischer P, Heinrich P, Sun X, Kemper J, von Bonin F, Perez-Rubio P, Taruttis F, Rehberg T, Dettmer K, Gronwald W, Reinders J, Engelmann JC, Dudek J, Klapper W, Trümper L, Spang R, Oefner PJ, Kube D
Nature communications 2018 Apr 17;9(1):1514

---

Gaseous signalling molecule SO2 via Hippo‑MST pathway to improve myocardial fibrosis of diabetic rats.

Liu M, Liu S, Tan W, Tang F, Long J, Li Z, Liang B, Chu C, Yang J
Molecular medicine reports 2017 Dec;16(6):8953-8963

---

Identification of Organ-Enriched Protein Biomarkers of Acute Liver Injury by Targeted Quantitative Proteomics of Blood in Acetaminophen- and Carbon-Tetrachloride-Treated Mouse Models and Acetaminophen Overdose Patients.

Qin S, Zhou Y, Gray L, Kusebauch U, McEvoy L, Antoine DJ, Hampson L, Park KB, Campbell D, Caballero J, Glusman G, Yan X, Kim TK, Yuan Y, Wang K, Rowen L, Moritz RL, Omenn GS, Pirmohamed M, Hood L
Journal of proteome research 2016 Oct 7;15(10):3724-3740

---

cMyc-mediated activation of serine biosynthesis pathway is critical for cancer progression under nutrient deprivation conditions.

Sun L, Song L, Wan Q, Wu G, Li X, Wang Y, Wang J, Liu Z, Zhong X, He X, Shen S, Pan X, Li A, Wang Y, Gao P, Tang H, Zhang H
Cell research 2015 Apr;25(4):429-44

---

Enhanced synthesis and diminished degradation of hydrogen sulfide in experimental colitis: a site-specific, pro-resolution mechanism.

Flannigan KL, Ferraz JG, Wang R, Wallace JL
PloS one 2013;8(8):e71962

Western blot

Supportive validation

Submitted by

Proteintech Group (provider)

Main image

Experimental details

The GOT1 antibody from Proteintech is a rabbit polyclonal antibody to a recombinant protein of human GOT1. This antibody recognizes human,mouse,rat antigen. The GOT1 antibody has been validated for the following applications: ELISA, WB analysis.

Appendix

14886-1-AP.pdf

Show more