PAB28562

antibody from Abnova Corporation

Targeting: ORAI3 MGC13024, TMEM142C

Western blot (Supportive data in Antibodypedia)

Immunocytochemistry (Supportive data in Antibodypedia)

Immunohistochemistry (Supportive data in Antibodypedia)

Antibody Data

Product number

PAB28562

Provider

Abnova Corporation

Product name

ORAI3 polyclonal antibody

Antibody type

Polyclonal

Description

Rabbit polyclonal antibody raised against recombinant ORAI3.

Antigen sequence

APLDTPTPMVPTSRVPGTLAPVATSLSPASNLPRS
SASAAPSQAEPACPPRQACGGGGAHGPGWQA


Isotype

IgG

Storage

Store at 4°C. For long term storage store at -20°C.Aliquot to avoid repeated freezing and thawing.

Western blot

Supportive validation

Submitted by

Abnova Corporation (provider)

Main image

Experimental details

Western blot analysis of Lane 1: Negative control (vector only transfected HEK293T lysate), Lane 2: Over-expression Lysate (Co-expressed with a C-terminal myc-DDK tag (~3.1 kDa) in mammalian HEK293T cells) with ORAI3 polyclonal antibody (PAB28562) at 1:100-1:250 dilution.

Immunocytochemistry

Supportive validation

Submitted by

Abnova Corporation (provider)

Main image

Experimental details

Immunofluorescent staining of A-431 with ORAI3 polyclonal antibody (Cat # PAB28562) at 1-4 ug/mL dilution shows positivity in nucleus but not nucleoli & cytoplasm.

Validation comment

Immunofluorescence

Immunohistochemistry

Supportive validation

Submitted by

Abnova Corporation (provider)

Main image

Experimental details

Immunohistochemical staining of human testis with ORAI3 polyclonal antibody (Cat # PAB28562) shows strong nuclear and cytoplasmic positivity in cells of seminiferus ducts at 1:50-1:200 dilution.

Validation comment

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)