PA5-109271

antibody from Invitrogen Antibodies

Targeting: ORAI3 MGC13024, TMEM142C

Western blot (Supportive data in Antibodypedia)

Immunocytochemistry (Supportive data in Antibodypedia)

Immunohistochemistry (Supportive data in Antibodypedia)

Flow cytometry (Supportive data in Antibodypedia)

Antibody Data

Product number

PA5-109271

Provider

Invitrogen Antibodies

Product name

ORAI3 Polyclonal Antibody

Antibody type

Polyclonal

Antigen

Synthetic peptide

Description

Positive Control: Raji, A549, MG-63, SH-SY-5Y, rat brain tissue, human liver cancer tissue, human kidney tissue, human placenta tissue, mouse testis tissue.

Reactivity

Human

Host

Rabbit

Isotype

IgG

Vial size

100 µL

Concentration

1 mg/mL

Storage

-20° C, Avoid Freeze/Thaw Cycles, store in dark

Western blot

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Western blot analysis of Orai3 in , Lane 1 : Raji cell lysates, Lane 2 : Raji cell lysates (anti-Orai3 antibody and immunizing peptide). Samples were incubated with Orai3 polyclonal antibody (Product # PA5-109271), at a dilution of 1:500.

Immunocytochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunofluorescent analysis of Orai3 in SH-SY-5Y cells (green). Samples were fixed in paraformaldehyde and permeabilised with 0.25% Triton X100/PBS, incubated with Orai3 polyclonal antibody (Product # PA5-109271), followed by DAPI (blue).

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunofluorescent analysis of Orai3 in MG-63 cells (green). Samples were fixed in paraformaldehyde and permeabilised with 0.25% Triton X100/PBS, incubated with Orai3 polyclonal antibody (Product # PA5-109271), followed by DAPI (blue).

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunofluorescent analysis of Orai3 in A549 cells (green). Samples were fixed in paraformaldehyde and permeabilised with 0.25% Triton X100/PBS, incubated with Orai3 polyclonal antibody (Product # PA5-109271), followed by DAPI (blue).

Immunohistochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemistry analysis of Orai3 in paraffin-embedded rat brain tissue. Samples were incubated with Orai3 polyclonal antibody (Product # PA5-109271), and followed by hematoxylin.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemistry analysis of Orai3 in paraffin-embedded rat brain tissue. Samples were incubated with Orai3 polyclonal antibody (Product # PA5-109271), and followed by hematoxylin.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemistry analysis of Orai3 in paraffin-embedded mouse testis tissue. Samples were incubated with Orai3 polyclonal antibody (Product # PA5-109271), and followed by hematoxylin.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemistry analysis of Orai3 in paraffin-embedded human placenta tissue. Samples were incubated with Orai3 polyclonal antibody (Product # PA5-109271), and followed by hematoxylin.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemistry analysis of Orai3 in paraffin-embedded human liver cancer tissue. Samples were incubated with Orai3 polyclonal antibody (Product # PA5-109271), and followed by hematoxylin.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemistry analysis of Orai3 in paraffin-embedded human kidney tissue. Samples were incubated with Orai3 polyclonal antibody (Product # PA5-109271), and followed by hematoxylin.

Flow cytometry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Flow cytometry of Orai3 in A549 cells (fuchsia) compared with an unlabelled control (cells without incubation with primary antibody; yellow). Samples were incubated with Orai3 polyclonal antibody (Product # PA5-109271) at a dilution of 1:100, followed by Alexa Fluor 488-conjugated goat anti-rabbit IgG.