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LearnH00005439-M02
antibody from Abnova Corporation
Targeting: POLR2J
hRPB14, POLR2J1, RPB11, RPB11A, RPB11m
Western blotAntibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [1]
- ELISA [1]
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Validation data
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- Product number
- H00005439-M02 - Provider product page
- Provider
- Abnova Corporation
- Proper citation
- Abnova Corporation Cat#H00005439-M02, RRID:AB_1578944
- Product name
- POLR2J monoclonal antibody (M02), clone 1A10
- Antibody type
- Monoclonal
- Description
- Mouse monoclonal antibody raised against a full-length recombinant POLR2J.
- Antigen sequence
MNAPPAFESFLLFEGEKKITINKDTKVPNACLFTI
NKEDHTLGNIIKSQLLKDPQVLFAGYKVPHPLEHK
IIIRVQTTPDYSPQEAFTNAITDLISELSLLEERF
RVAIKDKQEGIE- Isotype
- IgG
- Antibody clone number
- 1A10
- Storage
- Store at -20°C or lower. Aliquot to avoid repeated freezing and thawing.
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Supportive validation
- Submitted by
- Abnova Corporation (provider)
- Main image
- Experimental details
- Western Blot analysis of POLR2J expression in transfected 293T cell line by POLR2J monoclonal antibody (M02), clone 1A10.Lane 1: POLR2J transfected lysate(13.3 KDa).Lane 2: Non-transfected lysate.
Supportive validation
- Submitted by
- Abnova Corporation (provider)
- Main image
- Experimental details
- Detection limit for recombinant GST tagged POLR2J is 0.3 ng/ml as a capture antibody.
- Validation comment
- Sandwich ELISA (Recombinant protein)
- Protocol
- Protocol
