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Western blotAntibody data
- Antibody Data
- Antigen structure
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- Validations
- Western blot [2]
- Immunohistochemistry [2]
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- Product number
- R30410 - Provider product page
- Provider
- NSJ Bioreagents
- Product name
- uPAR Antibody uPA Receptor
- Antibody type
- Polyclonal
- Antigen
- An amino acid sequence from the C-terminus of human uPAR (CNHPDLDVQYRS) was used as the immunogen for this uPAR antibody.
- Description
- Antigen affinity purified antibody
- Reactivity
- Human
- Host
- Rabbit
- Conjugate
- Unconjugated
- Vial size
- 100 µg
- Concentration
- Lyophilized; resuspend with 200 ul for 0.5 mg/ml
- Storage
- After reconstitution, the uPAR antibody can be stored for up to one month at 4°C. For long-term, aliquot and store at -20°C. Avoid repeated freezing and thawing.
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Supportive validation
- Submitted by
- NSJ Bioreagents (provider)
- Main image
- Experimental details
- Western blot testing of uPAR antibody and Lane 1: MCF-7; 2: HeLa; 3: Raji; 4: SMMC-7721 cell lysate
- Submitted by
- NSJ Bioreagents (provider)
- Main image
- Experimental details
- Western blot testing of human HeLa cell lysate with uPAR antibody. Predicted molecular weight ~37 kDa but may be observed at higher molecular weights due to glycosylation.
Supportive validation
- Submitted by
- NSJ Bioreagents (provider)
- Main image
- Experimental details
- IHC-P: uPAR antibody testing of human breast cancer tissue
- Submitted by
- NSJ Bioreagents (provider)
- Main image
- Experimental details
- IHC staining of FFPE human glioma tissue with uPAR antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.
