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Western blotAntibody data
- Antibody Data
- Antigen structure
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- Validations
- Western blot [2]
- Immunocytochemistry [1]
- Immunohistochemistry [1]
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- Product number
- PA5-53243 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- DDX47 Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Recombinant full-length protein
- Description
- Immunogen sequence: IHRVGRTARA GRSGKAITFV TQYDVELFQR IEHLIGKKLP GFPTQDDEVM MLTERVAEAQ RFARMELREH GEKKKRSRED AGDNDDTEGA IGVRNKVAGG KMKKRKGR Highest antigen sequence identity to the following orthologs: Mouse - 94%, Rat - 94%.
- Reactivity
- Human
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 100 µL
- Concentration
- 0.05 mg/mL
- Storage
- Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of DDX47 in Lane 1: Marker (kDa) 230, 130, 95, 72, 56, 36, 28, 17, 11; Lane 2: Human cell line RT-4; Lane 3: Human cell line U-251MG sp. Samples were probed using a DDX47 Polyclonal Antibody (Product # PA5-53243).
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of DDX47 in Lane 1: NIH-3T3 cell lysate (Mouse embryonic fibroblast cells); Lane 2: NBT-II cell lysate (Rat Wistar bladder tumour cells). Samples were probed using a DDX47 Polyclonal Antibody (Product # PA5-53243).
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunofluorescent staining of DDX47 in human cell line U-2 OS shows positivity in nucleoli. Samples were probed using a DDX47 Polyclonal Antibody (Product # PA5-53243).
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemical staining of DDX47 in human esophogial tissue shows strong nuclear positivity in squamous epithelial cells. Samples were probed using a DDX47 Polyclonal Antibody (Product # PA5-53243).
