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Western blotAntibody data
- Antibody Data
- Antigen structure
- References [0]
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- Validations
- Western blot [2]
- Immunohistochemistry [4]
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Validation data
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- Product number
- PA5-48162 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- CLEC4F Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Synthetic peptide
- Reactivity
- Human
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 400 µL
- Concentration
- 0.5 mg/mL
- Storage
- Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of CLEC4F in various lysates. Samples were incubated with CLEC4F polyclonal antibody (Product # PA5-48162) using a dilution of 1:2,000 followed by Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at a dilution of 1:10,000. Lysates/proteins: 20 µg per lane. Lane 1: Hela whole cell lysate; Lane 2: U-2OS whole cell lysate. Predicted band size: 66 kDa. Blocking/Dilution buffer: 5% NFDM/TBST.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of CLEC4F in CCRF-CEM whole cell lysate. Samples were incubated with CLEC4F polyclonal antibody (Product # PA5-48162) using a dilution of 1:500 followed by Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at a dilution of 1:10,000. Lysates/proteins: 20 µg per lane. Predicted band size: 66 kDa. Blocking/Dilution buffer: 5% NFDM/TBST.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemistry analysis of CLEC4F in paraffin-embedded human skeletal muscle tissue. Samples were incubated with CLEC4F polyclonal antibody (Product # PA5-48162) using a dilution of 1:250 for 15 min at room temperature followed by Leica Bond Polymer Refine Detection. Tissue was fixed with formaldehyde at room temperature. Heat induced epitope retrieval was performed by EDTA buffer (pH 9 0).
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemistry analysis of CLEC4F in paraffin-embedded human liver tissue. Samples were incubated with CLEC4F polyclonal antibody (Product # PA5-48162) using a dilution of 1:250 for 15 min at room temperature followed by Leica Bond Polymer Refine Detection. Tissue was fixed with formaldehyde at room temperature. Heat induced epitope retrieval was performed by EDTA buffer (pH 9 0).
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemistry analysis of CLEC4F in paraffin-embedded human skeletal muscle tissue. Samples were incubated with CLEC4F polyclonal antibody (Product # PA5-48162) using a dilution of 1:250 for 15 min at room temperature followed by Leica Bond Polymer Refine Detection. Tissue was fixed with formaldehyde at room temperature. Heat induced epitope retrieval was performed by EDTA buffer (pH 9 0).
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemistry analysis of CLEC4F in paraffin-embedded human liver tissue. Samples were incubated with CLEC4F polyclonal antibody (Product # PA5-48162) using a dilution of 1:250 for 15 min at room temperature followed by Leica Bond Polymer Refine Detection. Tissue was fixed with formaldehyde at room temperature. Heat induced epitope retrieval was performed by EDTA buffer (pH 9 0).
