16064-1-AP

antibody from Proteintech Group

Targeting: PMPCB MPPB, MPPP52

Western blot (Supportive data in Antibodypedia)

ELISA (Data presented on provider website)

Immunocytochemistry (Supportive data in Antibodypedia)

Immunoprecipitation (Supportive data in Antibodypedia)

Immunohistochemistry (Supportive data in Antibodypedia)

Antibody Data

Product number

16064-1-AP

Provider

Proteintech Group

Proper citation

Proteintech Cat#16064-1-AP, RRID:AB_2167122

Product name

PMPCB antibody

Antibody type

Polyclonal

Description

KD/KO validated PMPCB antibody (Cat. #16064-1-AP) is a rabbit polyclonal antibody that shows reactivity with human, mouse, rat and has been validated for the following applications: IF, IHC, IP, WB,ELISA.

Reactivity

Human, Mouse, Rat

Host

Rabbit

Conjugate

Unconjugated

Isotype

IgG

Vial size

20ul, 150ul

References

Submitted references

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In Situ Peroxidase Labeling Followed by Mass-Spectrometry Reveals TIA1 Interactome.

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DELE1 tracks perturbed protein import and processing in human mitochondria.

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Nature communications 2022 Apr 6;13(1):1853

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Quantitative dSTORM super-resolution microscopy localizes Aurora kinase A/AURKA in the mitochondrial matrix.

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Mitochondrial Aurora kinase A induces mitophagy by interacting with MAP1LC3 and Prohibitin 2.

Bertolin G, Alves-Guerra MC, Cheron A, Burel A, Prigent C, Le Borgne R, Tramier M
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Cell reports 2021 Jun 29;35(13):109321

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Mitochondrial stress is relayed to the cytosol by an OMA1-DELE1-HRI pathway.

Guo X, Aviles G, Liu Y, Tian R, Unger BA, Lin YT, Wiita AP, Xu K, Correia MA, Kampmann M
Nature 2020 Mar;579(7799):427-432

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LRRK2 impairs PINK1/Parkin-dependent mitophagy via its kinase activity: pathologic insights into Parkinson's disease.

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Western blot

Supportive validation

Submitted by

Proteintech Group (provider)

Main image

Experimental details

MCF7 cells were subjected to SDS PAGE followed by western blot with 16064-1-AP(PMPCB antibody) at dilution of 1:500

Sample type

cell line

Immunocytochemistry

Supportive validation

Submitted by

Proteintech Group (provider)

Main image

Experimental details

The PMPCB antibody from Proteintech is a rabbit polyclonal antibody to a recombinant protein of human PMPCB. This antibody recognizes human,mouse,rat antigen. The PMPCB antibody has been validated for the following applications: ELISA, WB, IHC, IP, IF analysis.

Appendix

16064-1-AP.pdf

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Immunoprecipitation

Supportive validation

Submitted by

Proteintech Group (provider)

Main image

Experimental details

IP Result of anti-PMPCB (IP:16064-1-AP, 3ug; Detection:16064-1-AP 1:500) with MCF-7 cells lysate 2560ug.

Sample type

cell line

Immunohistochemistry

Supportive validation

Submitted by

Proteintech Group (provider)

Main image

Experimental details

Immunohistochemical of paraffin-embedded human lung cancer using 16064-1-AP(PMPCB antibody) at dilution of 1:100 (under 10x lens)

Sample type

tissue

Supportive validation

Submitted by

Proteintech Group (provider)

Main image

Experimental details

Immunohistochemical of paraffin-embedded human lung cancer using 16064-1-AP(PMPCB antibody) at dilution of 1:100 (under 40x lens)

Sample type

tissue