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- Antibody Data
- Antigen structure
- References [1]
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- Validations
- Western blot [1]
- Immunocytochemistry [1]
- Immunohistochemistry [3]
- Other assay [1]
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- Product number
- PA5-30217 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- RPL6 Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Recombinant protein fragment
- Description
- Recommended positive controls: A549, H1299, HCT116, MCF-7. Predicted reactivity: Mouse (91%), Rat (91%), Xenopus laevis (81%), Pig (94%), Chicken (83%), Rhesus Monkey (99%), Chimpanzee (100%), Bovine (95%). Store product as a concentrated solution. Centrifuge briefly prior to opening the vial.
- Reactivity
- Human, Mouse, Rat
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 100 µL
- Concentration
- 1 mg/mL
- Storage
- Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
Submitted references RNA-Seq Transcriptome Analysis of Differentiated Human Oligodendrocytic MO3.13 Cells Shows Upregulation of Genes Involved in Myogenesis.
Głowacka A, Kilańczyk E, Maksymowicz M, Zawadzka M, Leśniak W, Filipek A
International journal of molecular sciences 2022 May 25;23(11)
International journal of molecular sciences 2022 May 25;23(11)
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western Blot using RPL6 Polyclonal Antibody (Product # PA5-30217). Sample (30 µg of whole cell lysate). Lane A: A549. Lane B: H1299. Lane C: HCT116. Lane D: MCF-7. 12% SDS PAGE. RPL6 Polyclonal Antibody (Product # PA5-30217) diluted at 1:1,000.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunofluorescent analysis of RPL6 in methanol-fixed HeLa cells using a RPL6 polyclonal antibody (Product # PA5-30217) at a 1:500 dilution.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- RPL6 Polyclonal Antibody detects RPL6 protein at cytoplasm on human lung carcinoma by immunohistochemical analysis. Sample: Paraffin-embedded lung carcinoma. RPL6 Polyclonal Antibody (Product # PA5-30217) dilution: 1:500. Antigen Retrieval: EDTA based buffer, pH 8.0, 15 min.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- RPL6 Polyclonal Antibody detects RPL6 protein at cytosol on mouse stomach by immunohistochemical analysis. Sample: Paraffin-embedded mouse stomach. RPL6 Polyclonal Antibody (Product # PA5-30217) dilution: 1:500. Antigen Retrieval: EDTA based buffer, pH 8.0, 15 min.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- RPL6 Polyclonal Antibody detects RPL6 protein at cytosol on rat fore brain by immunohistochemical analysis. Sample: Paraffin-embedded rat fore brain. RPL6 Polyclonal Antibody (Product # PA5-30217) dilution: 1:500. Antigen Retrieval: EDTA based buffer, pH 8.0, 15 min.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Assessment of nucleolar integrity of MO3.13 cells. Control cells (Ctrl) and cells treated with PMA or CX-5461. Representative images after staining with specific antibodies against NPM1 (green, upper panel) and RPL6 (green, lower panel) and DAPI (cyan, both panels). Scale bar is 10 um.
