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Western blot
ImmunoprecipitationAntibody data
- Antibody Data
- Antigen structure
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- Validations
- Western blot [4]
- Immunohistochemistry [2]
- Other assay [1]
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- Product number
- A300-253A - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- EMSY Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Other
- Reactivity
- Human
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 100 µL
- Concentration
- 1 mg/mL
- Storage
- 4° C
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Detection of human EMSY by western blot. Samples: Whole cell lysate (50 µg) from BeWo, AlphaTC1 Clone 9, A20, 3T3-L1, and cells. Antibody: Affinity purified rabbit anti-EMSY antibody EMSY (Product # A300-253A lot 3) used for WB at 0.1 µg/mL. Detection: Chemiluminescence with an exposure time of 30 seconds.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Detection of human EMSY by western blot. Samples: Whole cell lysate (50 µg) from BeWo, AlphaTC1 Clone 9, A20, 3T3-L1, and cells. Antibody: Affinity purified rabbit anti-EMSY antibody EMSY (Product # A300-253A lot 3) used for WB at 0.1 µg/mL. Detection: Chemiluminescence with an exposure time of 30 seconds.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Detection of human EMSY by western blot of immunoprecipitates. Samples: Whole cell lysate (1 mg for IP; 20% of IP loaded) from HeLa cells. Antibodies: Affinity purified rabbit anti-EMSY antibody A300-253A (lot A300-253A-2) used for IP at 6 µg/mg lysate. EMSY was also immunoprecipitated by a previous lot of this antibody (lot A300-253A-1) and rabbit anti-EMSY antibody BL1049. For blotting immunoprecipitated EMSY, A300-253A was used at 1 µg/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Detection of human EMSY by western blot. Samples: Whole cell lysate (50 µg) from HeLa, 293T, Jurkat, mouse TCMK-1, and mouse NIH3T3 cells. Antibodies: Affinity purified rabbit anti-EMSY antibody A300-253A (lot A300-253A-2) used for WB at 0.1 µg/ml. Detection: Chemiluminescence with an exposure time of 3 minutes.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Detection of human EMSY by immunohistochemistry. Sample: FFPE section of human prostate carcinoma. Antibody: Affinity purified rabbit anti-EMSY (Product # A300-253A; lot 3) used at a dilution of 1:1,000 (1 µg/mL). Detection: DAB.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Detection of human EMSY by immunohistochemistry. Sample: FFPE section of human stomach carcinoma. Antibody: Affinity purified rabbit anti-EMSY (Cat. No. A300-253A Lot2) used at a dilution of 1:1,000 (1µg/ml). Detection: DAB.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Detection of human EMSY by western blot of immunoprecipitates. Samples: Whole cell lysate (1.0 mg per IP reaction; 5% of IP loaded) from Jurkat cells prepared using NETN lysis buffer. Antibodies: Affinity purified rabbit anti-EMSY antibody (Product # A300-253A lot 3) used for IP at 6 µg per reaction. EMSY was also immunoprecipitated by a previous lot of this antibody (A300-253A lot 2) and another antibody against a different epitope of EMSY (BL1049). For blotting immunoprecipitated EMSY (Product # A300-253A) was used at 0.1 µg/mL. Detection: Chemiluminescence with an exposure time of 30 seconds.
