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LearnPA5-29109
antibody from Invitrogen Antibodies
Targeting: ATP6V0A4
a4, ATP6N1B, ATP6N2, RDRTA2, RTA1C, RTADR, Stv1, Vph1, VPP2
Western blotAntibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [1]
- Immunocytochemistry [2]
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- Product number
- PA5-29109 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- ATP6V0A4 Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Recombinant protein fragment
- Description
- Recommended positive controls: 293T, A431, H1299, HeLa, Molt-4, Raji. Predicted reactivity: Mouse (89%), Bovine (84%). Store product as a concentrated solution. Centrifuge briefly prior to opening the vial.
- Reactivity
- Human
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 100 µL
- Concentration
- 0.65 mg/mL
- Storage
- Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western Blot using ATP6V0A4 Polyclonal Antibody (Product # PA5-29109). Sample (30 µg of whole cell lysate). Lane A: A431. Lane B: Molt-4 . 7.5% SDS PAGE. ATP6V0A4 Polyclonal Antibody (Product # PA5-29109) diluted at 1:1,000.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunofluorescent analysis of ATP6V0A4 showing staining in the cytoplasm of A431 cells. A431 cells were fixed in ice-cold MeOH for 5 min and stained using an ATP6V0A4 polyclonal antibody (Product # PA5-29109) diluted at 1:500. Blue: Hoechst 33342 staining.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- ATP6V0A4 Polyclonal Antibody detects ATP6V0A4 protein at cytoplasm by immunofluorescent analysis. Sample: A431 cells were fixed in ice-cold MeOH for 5 min. Green: ATP6V0A4 protein stained by ATP6V0A4 Polyclonal Antibody (Product # PA5-29109) diluted at 1:500. Blue: Hoechst 33342 staining.
