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- Western blot [1]
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- Product number
- AM32984PU-N - Provider product page
- Provider
- OriGene
- Product name
- CEACAM16 mouse monoclonal antibody, clone SU-9D5, Purified
- Antibody type
- Monoclonal
- Description
- CEACAM16 mouse monoclonal antibody, clone SU-9D5, Purified
- Host
- Mouse
- Conjugate
- Unconjugated
- Epitope
- CEACAM16
- Isotype
- IgG
- Antibody clone number
- SU-9D5
- Vial size
- 100 µg
- Concentration
- 1.0 mg/ml
Submitted references Loss of mammal-specific tectorial membrane component carcinoembryonic antigen cell adhesion molecule 16 (CEACAM16) leads to hearing impairment at low and high frequencies.
Kammerer R, Rüttiger L, Riesenberg R, Schäuble C, Krupar R, Kamp A, Sunami K, Eisenried A, Hennenberg M, Grunert F, Bress A, Battaglia S, Schrewe H, Knipper M, Schneider MR, Zimmermann W
The Journal of biological chemistry 2012 Jun 22;287(26):21584-98
The Journal of biological chemistry 2012 Jun 22;287(26):21584-98
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Supportive validation
- Submitted by
- OriGene (provider)
- Main image
- Experimental details
- SDS-PAGE analysis of purified SU-9D5 monoclonal antibody: Lane 1: molecular weight marker, Lane 2: 2 ug of purified SU-9D5 antibody. Proteins were separated by SDSPAGE and stained with RAPID StainTM Reagent.
- Validation comment
- WB
Supportive validation
- Submitted by
- OriGene (provider)
- Main image
- Experimental details
- Immunohistological staining using SU-9D5 ? on paraffin sections of cochlea tissue of an adult transgenic mouse with a human CEACAM16 gene (Ceacam16+/+ mouse cochlea). Binding of biotinylated SU-9D5 was detected with horseradish peroxidase-coupled streptavidin and stained with 3-amino-9-ethylcarbazole.
- Validation comment
- IHC
Supportive validation
- Submitted by
- OriGene (provider)
- Main image
- Experimental details
- FACS analysis of BOSC23 cells using SU-9D5 ?. BOSC23 cells were transiently transfected with an expression vector encoding either CEACAM16 (red curve) or an irrelevant protein (control transfectant). Binding of SU-9D5 was detected with a PE- conjugated secondary antibody. A positive signal was obtained only with CEACAM16 transfected cells.
- Validation comment
- FC
- Submitted by
- OriGene (provider)
- Main image
- Experimental details
- BOSC23 cells were transiently transfected with expression vectors containing either the cDNA of CEACAM1, CEACAM3, 4, 6, 8 19, or 20. Expression of the constructs was tested with monoclonal antibodies known to recognize the corresponding proteins (data not shown). An irrelevant monoclonal antibody served as a negative control (black curves). For specificity testing, protein G-purified SU-9D5 was tested on all CEACAM transfectants. A positive signal was obtained only with CEACAM16 transfected cells (red curve).
- Validation comment
- FC
