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Western blotAntibody data
- Antibody Data
- Antigen structure
- References [1]
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- Validations
- Western blot [4]
- Immunoprecipitation [1]
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- Product number
- NB100-77291 - Provider product page
- Provider
- Novus Biologicals
- Proper citation
- Novus Cat#NB100-77291, RRID:AB_1086114
- Product name
- Rabbit Polyclonal ZNF638 Antibody
- Antibody type
- Polyclonal
- Description
- Immunogen affinity purified.
- Reactivity
- Human, Mouse
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 0.1 ml
- Concentration
- 0.2 mg/ml
- Storage
- Store at 4C. Do not freeze.
Submitted references Regulation of adipocyte differentiation by the zinc finger protein ZNF638.
Meruvu S, Hugendubler L, Mueller E
The Journal of biological chemistry 2011 Jul 29;286(30):26516-23
The Journal of biological chemistry 2011 Jul 29;286(30):26516-23
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Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: ZNF638 Antibody [NB100-77291] - Detection of Human ZNF638/NP220 by Western Blot. Samples: Whole cell lysate (50 ug) from 293T cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-ZNF638/NP220 antibody NB100-77291 used for WB at 0.1 ug/ml. Detection: Chemiluminescence with an exposure time of 3 minutes.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: ZNF638 Antibody [NB100-77291] - Detection of Human ZNF638/NP220 on HeLa whole cell lysate using NB100-291. ZNF638/NP220 was also immunoprecipitated by another rabbit anti-ZNF638/NP220 antibody.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: ZNF638 Antibody [NB100-77291] - Whole cell lysate (50 ug) from HeLa, HEK293T, and Jurkat cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-ZNF638/NP220 antibody used for WB at 0.1 ug/ml. Detection: Chemiluminescence with an exposure time of 10 seconds.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: ZNF638 Antibody [NB100-77291] - Whole cell lysate (50 ug) from NIH 3T3 cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-ZNF638/NP220 antibodyused for WB at 0.1 ug/ml. Detection: Chemiluminescence with an exposure time of 75 seconds.
Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunoprecipitation: ZNF638 Antibody [NB100-77291] - Detection of human ZNF638/NP220 by western blot of immunoprecipitates. Samples: Whole cell lysate (1.0 mg per IP reaction; 20% of IP loaded) from HeLa cells prepared using NETN lysis buffer. Antibodies: Affinity purified rabbit anti-ZNF638/NP220 antibody NB100-77291 used for IP at 6 ug per reaction. ZNF638/NP220 was also immunoprecipitated by rabbit anti-ZNF638/NP220 antibody NB100-77290. For blotting immunoprecipitated ZNF638/NP220, NB100-77291 was used at 0.2 ug/ml. Detection: Chemiluminescence with an exposure time of 75 seconds.
