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Western blot
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- Antibody Data
- Antigen structure
- References [1]
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- Validations
- Other assay [1]
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- Product number
- PA5-31090 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- OAS3 Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Recombinant protein fragment
- Description
- Recommended positive controls: PC-3. Store product as a concentrated solution. Centrifuge briefly prior to opening the vial.
- Reactivity
- Human
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 100 µL
- Concentration
- 0.33 mg/mL
- Storage
- Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
Submitted references RNase L Amplifies Interferon Signaling by Inducing Protein Kinase R-Mediated Antiviral Stress Granules.
Manivannan P, Siddiqui MA, Malathi K
Journal of virology 2020 Jun 16;94(13)
Journal of virology 2020 Jun 16;94(13)
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Supportive validation
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- Invitrogen Antibodies (provider)
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- Experimental details
- FIG 5 Effect of avSG formation on IFN signaling. (A and B) HT1080 WT and G3BP1 KO cells were treated with IFN-beta (1,000 U/mL) for 24 h, and ISG15 and ISG56 mRNA levels were measured and normalized to those of GAPDH by qRT-PCR (A), or the cells were transfected with ISG15-luc or ISG56-luc reporter constructs, along with beta-galactosidase plasmids, and 24 h later treated with IFN-beta (1,000 U/mL), and luciferase activity was measured and normalized to beta-galactosidase levels (B). (C) WT, G3BP1 KO, and RNase L KO cells were treated with IFN-beta (1,000 U/mL) for the indicated times, and cell lysates were analyzed for phosphorylation of STAT1 and induction of OAS2, OAS3, and ISG56 in immunoblots. beta-Actin was used to normalize loading. (D) WT, G3BP1 KO, and RNase L KO cells were treated with IFN-beta (1,000 U/mL) for 16 h, and nuclear translocation of P-STAT1 was determined by immunofluorescence; nuclei were stained with DAPI. (Right) Quantification of P-STAT1 nuclear translocation from five random fields. The data represent means + SE for the results of three independent experiments. n.s, not significant.
