H00006901-M12
antibody from Abnova Corporation
Targeting: TAZ
BTHS, CMD3A, EFE, EFE2, G4.5, TAZ1, XAP-2
Antibody data
- Antibody Data
- Antigen structure
- References [3]
- Comments [0]
- Validations
- Western blot [1]
- ELISA [1]
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Validation data
Reference
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- Product number
- H00006901-M12 - Provider product page
- Provider
- Abnova Corporation
- Proper citation
- Abnova Corporation Cat#H00006901-M12, RRID:AB_733251
- Product name
- TAZ monoclonal antibody (M12), clone 1B10
- Antibody type
- Monoclonal
- Description
- Mouse monoclonal antibody raised against a full length recombinant TAZ.
- Antigen sequence
MPLHVKWPFPAVPPLTWTLASSVVMGLVGTYSCFW
TSEWAQAEAGPPGYPCPAGGILKLRHIWNLKLMRW
TPAAADICFTKELHSHFFSLGKCVPVCRGDGVYQK
GMDFILEKLNHGDWVHIFPEGIGRLIAECHLNPII
LPLWHVGEPGDGDREMASGVGGLGLPLVPGCPAPP
HVWPSVHCAAGMNDVLPNSPPYFPRFGQKITVLIG
KPFSALPVLERLRAENKSAVEMRKALTDFIQEEFQ
HLKTQAEQLHNHLQPGR- Isotype
- IgG
- Antibody clone number
- 1B10
- Storage
- Store at -20°C or lower. Aliquot to avoid repeated freezing and thawing.
Submitted references Involvement of YAP, TAZ and HSP90 in contact guidance and intercellular junction formation in corneal epithelial cells.
Role of substratum stiffness in modulating genes associated with extracellular matrix and mechanotransducers YAP and TAZ.
Substratum stiffness and latrunculin B modulate the gene expression of the mechanotransducers YAP and TAZ in human trabecular meshwork cells.
Raghunathan VK, Dreier B, Morgan JT, Tuyen BC, Rose BW, Reilly CM, Russell P, Murphy CJ
PloS one 2014;9(10):e109811
PloS one 2014;9(10):e109811
Role of substratum stiffness in modulating genes associated with extracellular matrix and mechanotransducers YAP and TAZ.
Raghunathan VK, Morgan JT, Dreier B, Reilly CM, Thomasy SM, Wood JA, Ly I, Tuyen BC, Hughbanks M, Murphy CJ, Russell P
Investigative ophthalmology & visual science 2013 Jan 14;54(1):378-86
Investigative ophthalmology & visual science 2013 Jan 14;54(1):378-86
Substratum stiffness and latrunculin B modulate the gene expression of the mechanotransducers YAP and TAZ in human trabecular meshwork cells.
Thomasy SM, Morgan JT, Wood JA, Murphy CJ, Russell P
Experimental eye research 2013 Aug;113:66-73
Experimental eye research 2013 Aug;113:66-73
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Supportive validation
- Submitted by
- Abnova Corporation (provider)
- Main image
- Experimental details
- TAZ monoclonal antibody (M12), clone 1B10. Western Blot analysis of TAZ expression in human kidney.
Supportive validation
- Submitted by
- Abnova Corporation (provider)
- Main image
- Experimental details
- Detection limit for recombinant GST tagged TAZ is approximately 3ng/ml as a capture antibody.
- Validation comment
- Sandwich ELISA (Recombinant protein)
- Protocol
- Protocol