PA5-81062

antibody from Invitrogen Antibodies

Targeting: TAZ BTHS, CMD3A, EFE, EFE2, G4.5, TAZ1, XAP-2

Western blot (Supportive data in Antibodypedia)

Immunoprecipitation (Data presented on provider website)

Immunohistochemistry (Supportive data in Antibodypedia)

Other assay (Supportive data in Antibodypedia)

Antibody Data

Product number

PA5-81062

Provider

Invitrogen Antibodies

Product name

TAZ Polyclonal Antibody

Antibody type

Polyclonal

Antigen

Synthetic peptide

Description

This product is preservative free. It is recommended to add sodium azide to avoid contamination (final concentration 0.05%-0.1%). This antibody has specificity for Human TAZ.

Reactivity

Human

Host

Rabbit

Isotype

IgG

Vial size

100 µL

Concentration

1.65 mg/mL

Storage

Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

Western blot

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Western blot analysis of TAZ in Lane A: A549 Whole Cell Lysate (30 µg). Samples were probed using a TAZ Polyclonal Antibody (Product # PA5-81062) at a 1:500 dilution, followed by a Goat Anti-Rabbit IgG (H+L), Dylight 800 Secondary Antibody at a 1:10000 dilution. Western blot was performed under reducing conditions. Predicted band size:44 kDa. Observed band size:40 kDa.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Western Blot using TAZ Polyclonal Antibody (Product # PA5-81062) at 1:500 dilution. Lane A: A549 Whole Cell Lysate. Lysates/proteins at 30 μg per lane. Secondary Goat Anti-Rabbit IgG H&L (DyLight™ 800) at 1:10,000 dilution. Developed using the Odyssey technique. Performed under reducing conditions. Predicted band size: 44 kDa. Observed band size: 40 kDa. (We are unsure of the identity of these extra bands).

Immunohistochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemical staining of human TAZ in human kidney with TAZ Polyclonal Antibody (Product # PA5-81062, 1:10,000, formalin-fixed paraffin embedded sections).

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemical staining of human TAZ in human placenta with TAZ Polyclonal Antibody (Product # PA5-81062, 1:10,000, formalin-fixed paraffin embedded sections).

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemical staining of human TAZ in human skeletal muscle with TAZ Polyclonal Antibody (Product # PA5-81062, 1:10,000, formalin-fixed paraffin embedded sections).

Other assay

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

TAZ Immunoprecipitation using: Lane A: 0.5 mg A549 Whole Cell Lysate 0.5 µL with TAZ Polyclonal Antibody (Product # PA5-81062) and 60 μg of Immunomagnetic beads Protein G. Primary antibody: TAZ Polyclonal Antibody, at 1:500 dilution. Secondary antibody: Dylight 800-labeled antibody to rabbit IgG (H+L), at 1:5,000 dilution. Developed using the Odyssey technique. Performed under reducing conditions. Predicted band size: 44 kDa. Observed band size: 44 kDa.