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- Antigen structure
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- Product number
- PA5-35924 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- MED13 Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Recombinant protein fragment
- Description
- Recommended positive controls: 293T, A431, HeLa, HepG2. Predicted reactivity: Mouse (94%), Rat (94%). Store product as a concentrated solution. Centrifuge briefly prior to opening the vial.
- Reactivity
- Human, Mouse
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 100 µL
- Concentration
- 1 mg/mL
- Storage
- Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
Submitted references The Mediator complex kinase module is necessary for fructose regulation of liver glycogen levels through induction of glucose-6-phosphatase catalytic subunit (G6pc).
Loss of MED12 activates the TGFβ pathway to promote chemoresistance and replication fork stability in BRCA-deficient cells.
Dual genome-wide CRISPR knockout and CRISPR activation screens identify mechanisms that regulate the resistance to multiple ATR inhibitors.
The subunit assembly state of the Mediator complex is nutrient-regulated and is dysregulated in a genetic model of insulin resistance and obesity.
Youn DY, Xiaoli AM, Zong H, Okada J, Liu L, Pessin J, Pessin JE, Yang F
Molecular metabolism 2021 Jun;48:101227
Molecular metabolism 2021 Jun;48:101227
Loss of MED12 activates the TGFβ pathway to promote chemoresistance and replication fork stability in BRCA-deficient cells.
Jackson LM, Dhoonmoon A, Hale A, Dennis KA, Schleicher EM, Nicolae CM, Moldovan GL
Nucleic acids research 2021 Dec 16;49(22):12855-12869
Nucleic acids research 2021 Dec 16;49(22):12855-12869
Dual genome-wide CRISPR knockout and CRISPR activation screens identify mechanisms that regulate the resistance to multiple ATR inhibitors.
Schleicher EM, Dhoonmoon A, Jackson LM, Clements KE, Stump CL, Nicolae CM, Moldovan GL
PLoS genetics 2020 Nov;16(11):e1009176
PLoS genetics 2020 Nov;16(11):e1009176
The subunit assembly state of the Mediator complex is nutrient-regulated and is dysregulated in a genetic model of insulin resistance and obesity.
Youn DY, Xiaoli AM, Kwon H, Yang F, Pessin JE
The Journal of biological chemistry 2019 Jun 7;294(23):9076-9083
The Journal of biological chemistry 2019 Jun 7;294(23):9076-9083
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of MED13 using A) 30 µg 293T whole cell lysate (B) 30 µg A431 whole cell lysate and C) 30 µg HeLa whole cell lysate. Samples were loaded onto a 5% SDS-PAGE gel and probed with a MED13 polyclonal antibody (Product # PA5-35924) at a dilution of 1:500.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western Blot analysis of MED13 was performed by separating 30 µg of various whole cell extracts by 5% SDS-PAGE. Proteins were transferred to a membrane and probed with a MED13 Polyclonal Antibody (Product # PA5-35924) at a dilution of 1:500 and a HRP-conjugated anti-rabbit IgG secondary antibody.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- NULL
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- NULL
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- NULL
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- NULL
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- NULL
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- NULL
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Figure 1 Glucose metabolic phenotype of the MED13-LKO mice. Twelve-week-old male Med13 fl/ fl and MED13-LKO mice were fed normal chow (LabDiet #5053) ad libitum as described in Methods (n = 8 per group). A) Representative Western blotting images of liver nuclear proteins (5 separate experiments performed) and relative densitometric analysis of the mediator complex kinase module subunits. B) Glycerol gradient column separation and shift of kinase module subunits to the size that correlates with different complexes shown on gels. Fractions 2-8 represent free subunits, 14-20 represent small complexes, and 24-27 represent large complexes according to the size standards. Five livers of MED13-LKO (left panel) or Med13 fl/ fl (right panel) mice that were fasted or fed with normal chow and 30% sucrose containing water were pooled and the same amount of liver protein lysate was loaded onto each column as described in Methods. C) Body weight and liver weight were measured in 12-week-old Med13 fl/ fl and MED13-LKO mice after 4-h normal chow ad libitum feeding. D) Respiratory exchange ratio (RER) over a 24-h period. E) Glucose tolerance test, F) insulin tolerance test, and G) pyruvate tolerance test for Med13 fl/ fl (red) and MED13-LKO (blue) mice were performed as described in Methods (n = 8 per group). P values were determined by Student''s t- test ( p < 0.05). Values are the mean +- SEM. Figure 1
