A301-277A

antibody from Invitrogen Antibodies

Targeting: MED13 KIAA0593, THRAP1, TRAP240

Western blot (Supportive data in Antibodypedia)

Immunoprecipitation (Recommended by provider)

Antibody Data

Product number

A301-277A

Provider

Invitrogen Antibodies

Product name

THRAP1/TRAP240 Polyclonal Antibody

Antibody type

Polyclonal

Antigen

Other

Description

The recommended shelf life for this product is 1 year from date of receipt.

Reactivity

Human

Host

Rabbit

Isotype

IgG

Vial size

100 µL

Concentration

0.20 mg/mL

Storage

4° C

Western blot

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Detection of human THRAP1/TRAP240 by western blot of immunoprecipitates. Samples: Whole cell lysate (0.5 or 1.0 mg per IP reaction; 20% of IP loaded) from HeLa cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-THRAP1/TRAP240 antibody A301-277A (lot A301-277A-2) used for IP at 6 µg per reaction. THRAP1/TRAP240 was also immunoprecipitated by a previous lot of this antibody (lot A301-277A-1) and rabbit anti-THRAP1/TRAP240 antibody A301-278A. For blotting immunoprecipitated THRAP1/TRAP240, A301-277A was used at 1 µg/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Detection of human THRAP1/TRAP240 by western blot. Samples: Whole cell lysate (50 µg) from HeLa and 293T cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-THRAP1/TRAP240 antibody A301-277A (lot A301-277A-2) used for WB at 0.1 µg/ml. Detection: Chemiluminescence with an exposure time of 3 minutes.