13474-1-AP

antibody from Proteintech Group

Targeting: CRY1 PHLL1

Western blot (Supportive data in Antibodypedia)

ELISA (Data presented on provider website)

Immunocytochemistry (Supportive data in Antibodypedia)

Immunohistochemistry (Data presented on provider website)

Antibody Data

Product number

13474-1-AP

Provider

Proteintech Group

Proper citation

Proteintech Cat#13474-1-AP, RRID:AB_10697652

Product name

Cryptochrome 1 antibody

Antibody type

Polyclonal

Description

Cryptochrome 1 antibody (Cat. #13474-1-AP) is a rabbit polyclonal antibody that shows reactivity with human, mouse, rat and has been validated for the following applications: IF, IHC, WB, ELISA.

Reactivity

Human, Mouse, Rat

Host

Rabbit

Conjugate

Unconjugated

Isotype

IgG

Vial size

20ul, 150ul

References

Submitted references

Circadian Clock Dysfunction Exacerbate Autistic-Like Behaviour and Wnt/β-Catenin Signalling Dysregulation in ASD Mice and Treatment of Melatonin.

Zhang Y, Chen Y, Li W, Tang L, Wang G, Li J, Feng X
Journal of cellular and molecular medicine 2026 Jan;30(1):e70991

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ZFP42 maintains stemness and rhythmic transcription in human epidermal stem and progenitor cells via CRY1.

Gao S, Tan H, Xu S, Zhang Z, Sun Y, Li Y, Jian D, Qi X, Tang Q, Chen R, Wang D, Zhen M, Wang P, Shu B, Li J
Communications biology 2026 Jan 21;9(1)

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Mini and enhanced CRISPR activators for cancer therapies.

Huang M, Wang K, Li A, Zhu X, Zhou Z, Yang C, Bi C, Zhang X
Journal of advanced research 2025 Sep;75:151-161

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Magnetogenetics inspired by animal Magnetoreception: ΔTRPV4(MagR) as a novel magnetogenetic actuator enabling remote neuromodulation of brain circuits.

Liu MN, Tian XY, Fang WC, Song R, Li F, Wang ZY, Lu GY, Wu N, Li J, Li H
Brain stimulation 2025 Sep-Oct;18(5):1455-1469

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Time-Restricted Feeding Affects Energy Metabolism in Lactating Striped Hamsters (Cricetulus barabensis, Cricetidae, Rodentia).

Li W, Dong X, He J, Jin X, Yin B, Bo T, Wen J
Biology 2025 Sep 12;14(9)

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Exploration of the mechanism underlying the impact of artificial light on guinea pig ovarian function via proteomics and acetylation analyses.

Shi J, Wang Y, Li L, Wang R, Peng Y, Zhou T, Hu D, Chen J, Tan Y
Ecotoxicology and environmental safety 2025 Sep 1;302:118719

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EZH2 expression in hepatocellular carcinoma and its relationship with circadian rhythm-related genes.

Wang X, He M, Zhou L, Chen W
Scientific reports 2025 Nov 26;15(1):42177

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Neuro-skeletal crosstalk: Brain-derived 5-HT mediates the bone-protective effects of β-Sitosterol against postmenopausal osteoporosis.

Wang K, Wang L, Zhou S, Chen X, Qian L, Zhang T, Sun X, Li M, Liu M, Guo Y, Hu Y, Ma Y
Phytomedicine : international journal of phytotherapy and phytopharmacology 2025 Nov 25;148:157248

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Loss of ARNTL Enhances ER Stress and Apoptosis in CKD Through Disruption of NRF2 Signaling.

He L, Zhang Q, Sheng X, Yin J, Yan Y, Wu M, Bao H, Hu S, Wang N, Cao A
FASEB journal : official publication of the Federation of American Societies for Experimental Biology 2025 Jun 15;39(11):e70715

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Dynamic and functional analyses of exosomal miRNAs regulating cellular microenvironment of ovarian cancer cells.

Wang Z, Huang Y, He S, Zhou Y, Zhao L, Wang F
Journal of ovarian research 2025 Feb 10;18(1):25

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Dysregulation of CRY1 impairs brain thyroid hormone pathway and promotes anxiety-like behavior in male mice.

Lv B, Liu Y, Yang Y, Wu Y, Chen Z, Zhang T, Yu F, Shi Y
Metabolism: clinical and experimental 2025 Aug;169:156292

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Involvement of circadian clock protein PER2 in controlling sleep deprivation induced HMGB1 up-regulation by targeting p300 in the cortex.

Zhang M, Ma Z, Cui H, Miao Y, Yin Y, Wen Q, Liu Z, Huang X, Xing C, Liu K, Peng H, Song L
Scientific reports 2025 Apr 10;15(1):12253

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Time of day differences in the regulation of glutathione levels in the rat lens.

Li B, Suzuki-Kerr H, Martis RM, Lim CJJ, Wang ZA, Nguyen TX, Donaldson PJ, Poulsen RC, Lim JC
Frontiers in ophthalmology 2024;4:1407582

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Sulforaphane Improves Liver Metabolism and Gut Microbiota in Circadian Rhythm Disorder Mice Models Fed With High-Fat Diets.

He C, Chen M, Jiang X, Ren J, Ganapathiraju SV, Lei P, Yang H, Pannu PR, Zhao Y, Zhang X
Molecular nutrition & food research 2024 Nov;68(21):e2400535

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Decreased Memory and Learning Ability Mediated by Bmal1/M1 Macrophages/Angptl2/Inflammatory Cytokine Pathway in Mice Exposed to Long-Term Blue Light Irradiation.

Hiramoto K, Kubo S, Tsuji K, Sugiyama D, Hamano H
Current issues in molecular biology 2024 May 18;46(5):4924-4934

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Alleviative effects of the parthenolide derivative ACT001 on insulin resistance induced by sodium propionate combined with a high-fat diet and its potential mechanisms.

Yu Q, Zuo X, Bai H, Zhang S, Luan J, Zhao Q, Zhao X, Feng X
European journal of pharmacology 2024 May 15;971:176529

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Cryptochrome 1 regulates ovarian granulosa cell senescence through NCOA4-mediated ferritinophagy.

Ma J, Chen S, Liu J, Liao Y, Li L, Wang CC, Song S, Feng R, Hu H, Quan S
Free radical biology & medicine 2024 May 1;217:1-14

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Chronotoxici-Plate Containing Droplet-Engineered Rhythmic Liver Organoids for Drug Toxicity Evaluation.

Zhou J, Huang YC, Wang W, Li J, Hou Y, Yi Z, Yang H, Hu K, Zhu Y, Wang Z, Ma S
Advanced science (Weinheim, Baden-Wurttemberg, Germany) 2024 Jul;11(28):e2305925

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Quercetin improved hepatic circadian rhythm dysfunction in middle-aged mice fed with vitamin D-deficient diet.

Li R, Wang G, Liu R, Luo L, Zhang Y, Wan Z
Journal of physiology and biochemistry 2024 Feb;80(1):137-147

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Metastatic effects of perfluorooctanoic acid (PFOA) on Drosophila melanogaster with metabolic reprogramming and dysrhythmia in a multigenerational exposure scenario.

Fangninou FF, Yu Z, Li W, Xue L, Yin D
The Science of the total environment 2024 Feb 20;912:169305

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Disruption of local circadian clocks in aristolochic acid-induced nephropathy in mice.

Xie D, Zhong S, Luo M, Xu J, Zheng R, Luo J, Wang Y, Guo Y, Guo L, Wu B, Lu D
Phytomedicine : international journal of phytotherapy and phytopharmacology 2024 Dec;135:156235

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Melatonin alleviates depression-like behaviors and cognitive dysfunction in mice by regulating the circadian rhythm of AQP4 polarization.

Yao D, Li R, Hao J, Huang H, Wang X, Ran L, Fang Y, He Y, Wang W, Liu X, Wang M
Translational psychiatry 2023 Oct 6;13(1):310

---

CRY1/2 regulate rhythmic CYP2A5 in mouse liver through repression of E4BP4.

Lin L, Huang Y, Wang J, Guo X, Yu F, He D, Wu C, Guo L, Wu B
Biochemical pharmacology 2023 Nov;217:115843

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Subtype-specific circadian clock dysregulation modulates breast cancer biology, invasiveness, and prognosis.

Hammarlund JA, Li SY, Wu G, Lian JW, Howell SJ, Clarke R, Adamson A, Gonçalves CF, Hogenesch JB, Meng QJ, Anafi RC
bioRxiv : the preprint server for biology 2023 May 19;

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Maternal circadian disruption before pregnancy impairs the ovarian function of female offspring in mice.

Guan Y, Xu M, Zhang Z, Liu C, Zhou J, Lin F, Fang J, Zhang Y, Yue Q, Zhen X, Yan G, Sun H, Liu W
The Science of the total environment 2023 Mar 15;864:161161

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Circadian clocks are modulated by compartmentalized oscillating translation.

Zhuang Y, Li Z, Xiong S, Sun C, Li B, Wu SA, Lyu J, Shi X, Yang L, Chen Y, Bao Z, Li X, Sun C, Chen Y, Deng H, Li T, Wu Q, Qi L, Huang Y, Yang X, Lin Y
Cell 2023 Jul 20;186(15):3245-3260.e23

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Sleep Fragmentation, Electroencephalographic Slowing, and Circadian Disarray in a Mouse Model for Intensive Care Unit Delirium.

Dulko E, Jedrusiak M, Osuru HP, Atluri N, Illendula M, Davis EM, Beenhakker MP, Lunardi N
Anesthesia and analgesia 2023 Jul 1;137(1):209-220

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Interaction of Bmal1 and eIF2α/ATF4 pathway was involved in Shuxie compound alleviation of circadian rhythm disturbance-induced hepatic endoplasmic reticulum stress.

Zhang M, Wu W, Huang C, Cai T, Wang M, Zhao N, Liu S, Yang S
Journal of ethnopharmacology 2023 Aug 10;312:116446

---

Acute hypoxia induced dysregulation of clock-controlled ovary functions.

Ding M, Lu Y, Huang X, Xing C, Hou S, Wang D, Zhang Y, Wang W, Zhang C, Zhang M, Meng F, Liu K, Liu G, Zhao J, Song L
Frontiers in physiology 2022;13:1024038

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Development of human cartilage circadian rhythm in a stem cell-chondrogenesis model.

Naven MA, Zeef LAH, Li S, Humphreys PA, Smith CA, Pathiranage D, Cain S, Woods S, Bates N, Au M, Wen C, Kimber SJ, Meng QJ
Theranostics 2022;12(8):3963-3976

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Dark-light cycle disrupts bone metabolism and suppresses joint deterioration in osteoarthritic rats.

Song X, Zhao M, Tang J, Ma T, Bai H, Wang X, Liu L, Li T, Xu X, Sheng X, Zhao B, Wang Y, Wang T, Guo Y, Zhang X, Gao L
Arthritis research & therapy 2022 Jun 28;24(1):158

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Melatonin regulates the immune response and improves Sjögren's syndrome-like symptoms in NOD/Ltj Mice.

Liu Y, Weng X, Wei M, Yu S, Ding Y, Cheng B
Biochemical pharmacology 2022 Jul;201:115073

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Capsaicin Attenuates Oleic Acid-Induced Lipid Accumulation via the Regulation of Circadian Clock Genes in HepG2 Cells.

Li R, Xiao J, Cao Y, Huang Q, Ho CT, Lu M
Journal of agricultural and food chemistry 2022 Jan 26;70(3):794-803

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Age-dependent effects of a high-fat diet combined with dietary advanced glycation end products on cognitive function and protection with voluntary exercise.

Luo L, Li R, Wang G, Chen J, Chen L, Qin LQ, Yu Z, Wan Z
Food & function 2022 Apr 20;13(8):4445-4458

---

hPER3 promotes adipogenesis via hHSP90AA1-mediated inhibition of Notch1 pathway.

Wan X, Zhu L, Zhao L, Peng L, Xiong J, Yang W, Yuan J, Liang F, Zhang K, Chen K
Cell death & disease 2021 Mar 19;12(4):301

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Decreased expression of the clock gene Bmal1 is involved in the pathogenesis of temporal lobe epilepsy.

Wu H, Liu Y, Liu L, Meng Q, Du C, Li K, Dong S, Zhang Y, Li H, Zhang H
Molecular brain 2021 Jul 14;14(1):113

---

Bromide impairs the circadian clock and glycolytic homeostasis via disruption of autophagy in rat H9C2 cardiomyocytes.

Jiang Y, Gu Y, Xu H, Tian X, Zhang X, Xu X, Yan W, Zhang X
BMC molecular and cell biology 2020 Jun 19;21(1):44

---

Hydrogen peroxide modulates clock gene expression via PRX2-STAT3-REV-ERBα/β pathway.

Ji G, Lv K, Chen H, Wang Y, Zhang Y, Li Y, Qu L
Free radical biology & medicine 2019 Dec;145:312-320

---

Jiao-tai-wan Up-regulates Hypothalamic and Peripheral Circadian Clock Gene Cryptochrome and Activates PI3K/AKT Signaling in Partially Sleep-deprived Rats.

Huang WY, Zou X, Lu FE, Su H, Zhang C, Ren YL, Fang K, Xu LJ, Wang KF, Chen QJ, Dong H
Current medical science 2018 Aug;38(4):704-713

---

SCA1(+) Cells from the Heart Possess a Molecular Circadian Clock and Display Circadian Oscillations in Cellular Functions.

Du Pré BC, Demkes EJ, Feyen DAM, Dierickx P, Crnko S, Kok BJM, Sluijter JPG, Doevendans PA, Vos MA, Van Veen TAB, Van Laake LW
Stem cell reports 2017 Sep 12;9(3):762-769

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Circadian networks in human embryonic stem cell-derived cardiomyocytes.

Dierickx P, Vermunt MW, Muraro MJ, Creyghton MP, Doevendans PA, van Oudenaarden A, Geijsen N, Van Laake LW
EMBO reports 2017 Jul;18(7):1199-1212

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Effect of Jiaotai Pill () on intestinal damage in partially sleep deprived rats.

Huang WY, Zou X, Lu FE, Zhang C, Ren YL, Xu LJ, Wang KF, Dong H
Chinese journal of integrative medicine 2017 Dec;23(12):901-907

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Rhythmic expression of miR-27b-3p targets the clock gene Bmal1 at the posttranscriptional level in the mouse liver.

Zhang W, Wang P, Chen S, Zhang Z, Liang T, Liu C
FASEB journal : official publication of the Federation of American Societies for Experimental Biology 2016 Jun;30(6):2151-60

Western blot

Supportive validation

Submitted by

Proteintech Group (provider)

Main image

Experimental details

Y79 cells were subjected to SDS PAGE followed by western blot with 13474-1-AP(CRY1 antibody) at dilution of 1:1000

Sample type

cell line

Immunocytochemistry

Supportive validation

Submitted by

Proteintech Group (provider)

Main image

Experimental details

Immunofluorescent analysis of Hela cells, using CRY1 antibody 13474-1-AP at 1:25 dilution and Rhodamine-labeled goat anti-rabbit IgG (red). Blue pseudocolor = DAPI (fluorescent DNA dye).

Sample type

cell line