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Western blot
ImmunoprecipitationAntibody data
- Antibody Data
- Antigen structure
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- Validations
- Western blot [2]
- Other assay [1]
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- Product number
- A301-257A - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- TLK2 Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Other
- Reactivity
- Human
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 100 µL
- Concentration
- 1 mg/mL
- Storage
- 4° C
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Detection of human TLK2 by western blot. Samples: Whole cell lysate from HEK293T (50, 15 and 5 µg) and HeLa (50 µg) cells. Antibody: Affinity purified rabbit anti-TLK2 antibody (Product # A301-257A lot 1) used for WB at 0.4 µg/mL. Detection: Chemiluminescence with an exposure time of 30 seconds.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Detection of human TLK2 by western blot and immunoprecipitation. Samples: Whole cell lysate from 293T (5, 15 and 50 µg for WB; 1 mg for IP, 20% of IP loaded) and HeLa (H; 50 µg) cells. Antibodies: Affinity purified rabbit anti-TLK2 antibody A301-257A used for WB at 0.4 µg/ml (A) and 1 µg/ml (B) and used for IP at 3 µg/mg lysate. TLK2 was also immunoprecipitated by rabbit anti-TLK2 antibody BL2513, which recognizes a downstream epitope. Detection: Chemiluminescence with exposure times of 30 seconds (A) and 10 seconds (B).
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Detection of human TLK2 by western blot of immunoprecipitates. Samples: Whole cell lysate (1.0 mg per IP reaction; 20% of IP loaded) from HEK293T cells prepared using NETN lysis buffer. Antibodies: Affinity purified rabbit anti-TLK2 antibody (Product # A301-257A; Lot 1) used for IP at 6 µg per reaction. TLK2 was also immunoprecipitated by a second antibody against a different epitope of TLK2 (BL2513). For blotting immunoprecipitated TLK2, Product # A301-257A was used at 1 µg/mL. Detection: Chemiluminescence with an exposure time of 10 seconds.
