- MA5-15108 - Invitrogen Antibodies MAPT antibody

Submitted references Negative Regulator of Ubiquitin-Like Protein 1 modulates the autophagy-lysosomal pathway via p62 to facilitate the extracellular release of tau following proteasome impairment.

Guarascio R, Salih D, Yasvoina M, Edwards FA, Cheetham ME, van der Spuy J
Human molecular genetics 2020 Jan 1;29(1):80-96

Silver nanoparticles exhibit coating and dose-dependent neurotoxicity in glutamatergic neurons derived from human embryonic stem cells.

Begum AN, Aguilar JS, Elias L, Hong Y
Neurotoxicology 2016 Dec;57:45-53

HMGB1, a pathogenic molecule that induces neurite degeneration via TLR4-MARCKS, is a potential therapeutic target for Alzheimer's disease.

Fujita K, Motoki K, Tagawa K, Chen X, Hama H, Nakajima K, Homma H, Tamura T, Watanabe H, Katsuno M, Matsumi C, Kajikawa M, Saito T, Saido T, Sobue G, Miyawaki A, Okazawa H
Scientific reports 2016 Aug 25;6:31895

LiCl attenuates thapsigargin-induced tau hyperphosphorylation by inhibiting GSK-3β in vivo and in vitro.

Fu ZQ, Yang Y, Song J, Jiang Q, Lin ZC, Wang Q, Zhu LQ, Wang JZ, Tian Q
Journal of Alzheimer's disease : JAD 2010;21(4):1107-17

LiCl attenuates thapsigargin-induced tau hyperphosphorylation by inhibiting GSK-3β in vivo and in vitro.

Fu ZQ, Yang Y, Song J, Jiang Q, Lin ZC, Wang Q, Zhu LQ, Wang JZ, Tian Q
Journal of Alzheimer's disease : JAD 2010;21(4):1107-17

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Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Western blot analysis of Tau was performed by loading 20 µg of brain homogenates from 1 month old mice (each lane contains homogenate from a independent mouse) per well onto an SDS-PAGE gel. Proteins were transferred to a nitrocellulose membrane and blocked with 5% milk in TBS for 1 hour. The membrane was probed with a Tau monoclonal antibody (Product # MA5-15108) at a dilution of 1:1000 at 4C overnight, washed in TBS-T, and probed with an HRP-conjugated anti-mouse IgG secondary antibody at a dilution of 1:2000 for 2 hours at room temperature. Detection was performed using ECL substrate (Product # 32106). Data courtesy of the Innovators Program.

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Western blot analysis of Tau was performed by loading 20 µg of brain homogenates from 1 month old mice (each lane contains homogenate from a independent mouse) per well onto an SDS-PAGE gel. Proteins were transferred to a nitrocellulose membrane and blocked with 5% milk in TBS for 1 hour. The membrane was probed with a Tau monoclonal antibody (Product # MA5-15108) at a dilution of 1:1000 at 4C overnight, washed in TBS-T, and probed with an HRP-conjugated anti-mouse IgG secondary antibody at a dilution of 1:2000 for 2 hours at room temperature. Detection was performed using ECL substrate (Product # 32106). Data courtesy of the Innovators Program.

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Western blot analysis of Tau in extracts from mouse and rat brain using Tau monoclonal antibody (Product # MA5-15108).

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Western blot was performed using Anti-Tau Monoclonal Antibody (S.125.0)(Product # MA5-15108) and 40-80 kDa bands corresponding to Tau were observed in Mouse and Rat Brain but not in Mouse Kidney or Mouse and Rat Lung. Tissue extracts (30 µg lysate) of Mouse Brain (Lane 1), Rat Brain (Lane 2), Mouse Kidney (Lane 3), Mouse Lung (Lane 4) or Rat Lung (Lane 5) were electrophoresed using NuPAGE™ 10% Bis-Tris Protein Gel (Product # NP0301BOX). Resolved proteins were then transferred onto a Nitrocellulose membrane (Product # IB23001) by iBlot® 2 Dry Blotting System (Product # IB21001). The blot was probed with the primary antibody (1:500) and detected by chemiluminescence with Goat anti-Mouse IgG (H+L) Superclonal™ Recombinant Secondary Antibody, HRP (Product # A28177,1:4000) using the iBright FL 1000 (Product # A32752).

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Immunofluorescent analysis of Tau in mouse dentate gyrus using a Tau monoclonal antibody (Product # MA5-15108) (red) and a CREB monoclonal antibody (blue).

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Immunofluorescent analysis of Tau in human Alzheimer brain using a Tau monoclonal antibody (Product # MA5-15108) (green) and an APP antibody (red).

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Immunofluorescent analysis of Tau using a monoclonal antibody (Product # MA5-15108).

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Immunohistochemical analysis of Tau in paraffin-embedded Alzheimer's brain using a Tau monoclonal antibody (Product # MA5-15108).

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Figure 2 Ser46 phosphorylation of MARCKS is a marker of degenerative neurites. (a) Anti-pMARCKS (Ser46)-stained amyloid plaque-like structures in the cerebrum of 5xFAD mice but not in wild type B6/SJL mice. The synthetic peptide ENGHVKVNGDA(pS)PA was used for antigen absorption. (b) Three-dimensional reconstruction of Abeta (green), Ser46-pMARCKS (red), and DAPI (blue) co-staining revealed that Ser46-phosphorylated MARCKS surrounds amyloid aggregates after cell death. The corresponding movie is attached as Supplementary Information . (c) Three-dimensional reconstruction of Abeta (green), Ser46-pMARCKS (red), and DAPI (blue) revealed similar patterns in human AD brains. (d) Three-dimensional reconstruction of tau (green), Ser46-pMARCKS (red), and DAPI (blue) revealed that Ser46-phosphorylation of MARCKS occurs in the axons of human AD brains.

MA5-15108

Antibody data