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antibody from Invitrogen Antibodies
Targeting: MAPT
DDPAC, FLJ31424, FTDP-17, MAPTL, MGC138549, MSTD, MTBT1, MTBT2, PPND, PPP1R103, tau
Western blot
ELISAAntibody data
- Antibody Data
- Antigen structure
- References [14]
- Comments [0]
- Validations
- Western blot [2]
- Immunohistochemistry [1]
- Other assay [5]
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- Product number
- 13-1400 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- Tau Monoclonal Antibody (T14)
- Antibody type
- Monoclonal
- Antigen
- Other
- Reactivity
- Human, Bovine
- Host
- Mouse
- Isotype
- IgG
- Antibody clone number
- T14
- Vial size
- 100 µg
- Storage
- -20°C
Submitted references Tau knockout exacerbates degeneration of parvalbumin-positive neurons in substantia nigra pars reticulata in Parkinson's disease-related α-synuclein A53T mice.
Adult-onset focal expression of mutated human tau in the hippocampus impairs spatial working memory of rats.
Focal expression of mutated tau in entorhinal cortex neurons of rats impairs spatial working memory.
Wnt-pathway activation during the early stage of neurodegeneration in FTDP-17 mice.
Age-dependent neurofibrillary tangle formation, neuron loss, and memory impairment in a mouse model of human tauopathy (P301L).
Microtubule-associated protein tau: a marker of paclitaxel sensitivity in breast cancer.
Increased tau phosphorylation on mitogen-activated protein kinase consensus sites and cognitive decline in transgenic models for Alzheimer's disease and FTDP-17: evidence for distinct molecular processes underlying tau abnormalities.
Formation of neurofibrillary tangles in P301l tau transgenic mice induced by Abeta 42 fibrils.
Characterization of mouse dishevelled (Dvl) proteins in Wnt/Wingless signaling pathway.
Pathogenic implications of mutations in the tau gene in pallido-ponto-nigral degeneration and related neurodegenerative disorders linked to chromosome 17.
Pathogenic implications of mutations in the tau gene in pallido-ponto-nigral degeneration and related neurodegenerative disorders linked to chromosome 17.
The neuropathology of a chromosome 17-linked autosomal dominant parkinsonism and dementia ("pallido-ponto-nigral degeneration").
The neuropathology of a chromosome 17-linked autosomal dominant parkinsonism and dementia ("pallido-ponto-nigral degeneration").
Cognitive, neuroimaging, and pathological studies in a patient with Pick's disease.
Jiao L, Zheng M, Duan J, Wu T, Li Z, Liu L, Xiang X, Tang X, He J, Li X, Zhang G, Ding J, Cai H, Lin X
FASEB journal : official publication of the Federation of American Societies for Experimental Biology 2020 Sep;34(9):12239-12254
FASEB journal : official publication of the Federation of American Societies for Experimental Biology 2020 Sep;34(9):12239-12254
Adult-onset focal expression of mutated human tau in the hippocampus impairs spatial working memory of rats.
Mustroph ML, King MA, Klein RL, Ramirez JJ
Behavioural brain research 2012 Jul 15;233(1):141-8
Behavioural brain research 2012 Jul 15;233(1):141-8
Focal expression of mutated tau in entorhinal cortex neurons of rats impairs spatial working memory.
Ramirez JJ, Poulton WE, Knelson E, Barton C, King MA, Klein RL
Behavioural brain research 2011 Jan 1;216(1):332-40
Behavioural brain research 2011 Jan 1;216(1):332-40
Wnt-pathway activation during the early stage of neurodegeneration in FTDP-17 mice.
Wiedau-Pazos M, Wong E, Solomon E, Alarcon M, Geschwind DH
Neurobiology of aging 2009 Jan;30(1):14-21
Neurobiology of aging 2009 Jan;30(1):14-21
Age-dependent neurofibrillary tangle formation, neuron loss, and memory impairment in a mouse model of human tauopathy (P301L).
Ramsden M, Kotilinek L, Forster C, Paulson J, McGowan E, SantaCruz K, Guimaraes A, Yue M, Lewis J, Carlson G, Hutton M, Ashe KH
The Journal of neuroscience : the official journal of the Society for Neuroscience 2005 Nov 16;25(46):10637-47
The Journal of neuroscience : the official journal of the Society for Neuroscience 2005 Nov 16;25(46):10637-47
Microtubule-associated protein tau: a marker of paclitaxel sensitivity in breast cancer.
Rouzier R, Rajan R, Wagner P, Hess KR, Gold DL, Stec J, Ayers M, Ross JS, Zhang P, Buchholz TA, Kuerer H, Green M, Arun B, Hortobagyi GN, Symmans WF, Pusztai L
Proceedings of the National Academy of Sciences of the United States of America 2005 Jun 7;102(23):8315-20
Proceedings of the National Academy of Sciences of the United States of America 2005 Jun 7;102(23):8315-20
Increased tau phosphorylation on mitogen-activated protein kinase consensus sites and cognitive decline in transgenic models for Alzheimer's disease and FTDP-17: evidence for distinct molecular processes underlying tau abnormalities.
Lambourne SL, Sellers LA, Bush TG, Choudhury SK, Emson PC, Suh YH, Wilkinson LS
Molecular and cellular biology 2005 Jan;25(1):278-93
Molecular and cellular biology 2005 Jan;25(1):278-93
Formation of neurofibrillary tangles in P301l tau transgenic mice induced by Abeta 42 fibrils.
Götz J, Chen F, van Dorpe J, Nitsch RM
Science (New York, N.Y.) 2001 Aug 24;293(5534):1491-5
Science (New York, N.Y.) 2001 Aug 24;293(5534):1491-5
Characterization of mouse dishevelled (Dvl) proteins in Wnt/Wingless signaling pathway.
Lee JS, Ishimoto A, Yanagawa S
The Journal of biological chemistry 1999 Jul 23;274(30):21464-70
The Journal of biological chemistry 1999 Jul 23;274(30):21464-70
Pathogenic implications of mutations in the tau gene in pallido-ponto-nigral degeneration and related neurodegenerative disorders linked to chromosome 17.
Clark LN, Poorkaj P, Wszolek Z, Geschwind DH, Nasreddine ZS, Miller B, Li D, Payami H, Awert F, Markopoulou K, Andreadis A, D'Souza I, Lee VM, Reed L, Trojanowski JQ, Zhukareva V, Bird T, Schellenberg G, Wilhelmsen KC
Proceedings of the National Academy of Sciences of the United States of America 1998 Oct 27;95(22):13103-7
Proceedings of the National Academy of Sciences of the United States of America 1998 Oct 27;95(22):13103-7
Pathogenic implications of mutations in the tau gene in pallido-ponto-nigral degeneration and related neurodegenerative disorders linked to chromosome 17.
Clark LN, Poorkaj P, Wszolek Z, Geschwind DH, Nasreddine ZS, Miller B, Li D, Payami H, Awert F, Markopoulou K, Andreadis A, D'Souza I, Lee VM, Reed L, Trojanowski JQ, Zhukareva V, Bird T, Schellenberg G, Wilhelmsen KC
Proceedings of the National Academy of Sciences of the United States of America 1998 Oct 27;95(22):13103-7
Proceedings of the National Academy of Sciences of the United States of America 1998 Oct 27;95(22):13103-7
The neuropathology of a chromosome 17-linked autosomal dominant parkinsonism and dementia ("pallido-ponto-nigral degeneration").
Reed LA, Schmidt ML, Wszolek ZK, Balin BJ, Soontornniyomkij V, Lee VM, Trojanowski JQ, Schelper RL
Journal of neuropathology and experimental neurology 1998 Jun;57(6):588-601
Journal of neuropathology and experimental neurology 1998 Jun;57(6):588-601
The neuropathology of a chromosome 17-linked autosomal dominant parkinsonism and dementia ("pallido-ponto-nigral degeneration").
Reed LA, Schmidt ML, Wszolek ZK, Balin BJ, Soontornniyomkij V, Lee VM, Trojanowski JQ, Schelper RL
Journal of neuropathology and experimental neurology 1998 Jun;57(6):588-601
Journal of neuropathology and experimental neurology 1998 Jun;57(6):588-601
Cognitive, neuroimaging, and pathological studies in a patient with Pick's disease.
Lieberman AP, Trojanowski JQ, Lee VM, Balin BJ, Ding XS, Greenberg J, Morrison D, Reivich M, Grossman M
Annals of neurology 1998 Feb;43(2):259-65
Annals of neurology 1998 Feb;43(2):259-65
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of Tau was performed by loading 20 µg of SHSY-5Y (lane1), Hep G2 (lane2), COLO205 (lane3), MCF7 (lane4) and Jurkat (lane5) cell lysates using Novex® NuPAGE® 4-12 % Bis-Tris gel (Product # NP0322BOX), XCell SureLock™ Electrophoresis System (Product # EI0002), Novex® Sharp Pre-Stained Protein Standard (LC5800), and iBlot® 2 Dry Blotting System (IB21001). Proteins were transferred to a nitrocellulose membrane and blocked with 5 % skim milk at 4ºC overnight. Tau was detected at ~ 78 kDa using Tau Mouse Monoclonal Antibody (Product # 13-1400) at 2-3 µg/mL in 5 % skim milk for 3 hours at room temperature on a rocking platform. Goat Anti-Mouse - HRP Secondary Antibody (Product # 62-6520) at 1:4000 dilution was used and chemiluminescent detection was performed using Pierce™ ECL Western Blotting Substrate (Product # 32106).
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of Tau was performed by loading 20 µg of SHSY-5Y (lane1), Hep G2 (lane2), COLO205 (lane3), MCF7 (lane4) and Jurkat (lane5) cell lysates using Novex® NuPAGE® 4-12 % Bis-Tris gel (Product # NP0322BOX), XCell SureLock™ Electrophoresis System (Product # EI0002), Novex® Sharp Pre-Stained Protein Standard (LC5800), and iBlot® 2 Dry Blotting System (IB21001). Proteins were transferred to a nitrocellulose membrane and blocked with 5 % skim milk at 4ºC overnight. Tau was detected at ~ 78 kDa using Tau Mouse Monoclonal Antibody (Product # 13-1400) at 2-3 µg/mL in 5 % skim milk for 3 hours at room temperature on a rocking platform. Goat Anti-Mouse - HRP Secondary Antibody (Product # 62-6520) at 1:4000 dilution was used and chemiluminescent detection was performed using Pierce™ ECL Western Blotting Substrate (Product # 32106).
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemical staining of Alzheimer's disease brain tissue using Ms anti-Tau (Product # 13-1400).
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- NULL
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- NULL
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- NULL
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- NULL
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- NULL
