44-751G
antibody from Invitrogen Antibodies
Targeting: MAPT
DDPAC, FLJ31424, FTDP-17, MAPTL, MGC138549, MSTD, MTBT1, MTBT2, PPND, PPP1R103, tau
Antibody data
- Antibody Data
- Antigen structure
- References [2]
- Comments [0]
- Validations
- Western blot [3]
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Validation data
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- Product number
- 44-751G - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- Phospho-Tau (Ser356) Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Synthetic peptide
- Reactivity
- Human, Mouse, Rat
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 100 µL
- Storage
- -20°C
Submitted references A proteomic analysis of MCLR-induced neurotoxicity: implications for Alzheimer's disease.
Developmental regulation of tau phosphorylation, tau kinases, and tau phosphatases.
Li G, Cai F, Yan W, Li C, Wang J
Toxicological sciences : an official journal of the Society of Toxicology 2012 Jun;127(2):485-95
Toxicological sciences : an official journal of the Society of Toxicology 2012 Jun;127(2):485-95
Developmental regulation of tau phosphorylation, tau kinases, and tau phosphatases.
Yu Y, Run X, Liang Z, Li Y, Liu F, Liu Y, Iqbal K, Grundke-Iqbal I, Gong CX
Journal of neurochemistry 2009 Mar;108(6):1480-94
Journal of neurochemistry 2009 Mar;108(6):1480-94
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Up-regulation and Antibody-Peptide Competition. Human recombinant Tau treated with PKA (36 µg per µg Tau) for 45 minutes and added to background extracts were resolved by SDS-PAGE on a 10% Tris-glycine gel and transferred to PVDF. The membrane was blocked with a 5% BSA-TBST buffer for one hour at room temperature, then incubated with the Tau (pS356) antibody in a 1% BSA-TBST buffer for two hours at room temperature, following prior incubation with: no peptide (1), the non-phosphopeptide corresponding to the phosphopeptide immunogen (2), a generic phosphoserine-containing peptide (3), or the phosphopeptide immunogen (4). After washing, the membrane was incubated with goat F (ab')2 anti-rabbit IgG HRP conjugate (Product # ALI4404) and signals were detected using the Pierce SuperSignal™ method. The data show that only the phosphopeptide corresponding to Tau (pS356) blocks the antibody signal, demonstrating the specificity of the antibody. This antibody does not cross-react with Tau (pS262) (data not shown).
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Up-regulation and Antibody-Peptide Competition. Human recombinant Tau treated with PKA (36 µg per µg Tau) for 45 minutes and added to background extracts were resolved by SDS-PAGE on a 10% Tris-glycine gel and transferred to PVDF. The membrane was blocked with a 5% BSA-TBST buffer for one hour at room temperature, then incubated with the Tau (pS356) antibody in a 1% BSA-TBST buffer for two hours at room temperature, following prior incubation with: no peptide (1), the non-phosphopeptide corresponding to the phosphopeptide immunogen (2), a generic phosphoserine-containing peptide (3), or the phosphopeptide immunogen (4). After washing, the membrane was incubated with goat F (ab')2 anti-rabbit IgG HRP conjugate (Product # ALI4404) and signals were detected using the Pierce SuperSignal™ method. The data show that only the phosphopeptide corresponding to Tau (pS356) blocks the antibody signal, demonstrating the specificity of the antibody. This antibody does not cross-react with Tau (pS262) (data not shown).
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis was performed on whole cell extracts (30 µg lysate) of Mouse Brain (Lane 1), and Rat Brain (Lane 2). The blots were probed with Anti-Phospho-Tau pSer356 Rabbit Polyclonal Antibody (Product # 44-751G, 1-3 µg/mL) and detected by chemiluminescence using Goat anti-Rabbit IgG (H+L) Secondary Antibody, HRP conjugate (Product # G-21234, 1:5000 dilution). A ~ 36 kDa band corresponding to Tau pSer356 was observed across cell lines tested. Known quantity of protein samples were electrophoresed using Novex® NuPAGE® 12 % Bis-Tris gel (Product # NP0342BOX), XCell SureLock™ Electrophoresis System (Product # EI0002) and Novex® Sharp Pre-Stained Protein Standard (Product # LC5800). Resolved proteins were then transferred onto a nitrocellulose membrane with iBlot® 2 Dry Blotting System (Product # IB21001). The membrane was probed with the relevant primary and secondary Antibody following blocking with 5 % skimmed milk. Chemiluminescent detection was performed using Pierce™ ECL Western Blotting Substrate (Product # 32106).