823902

antibody from BioLegend

Targeting: MAPT DDPAC, FLJ31424, FTDP-17, MAPTL, MGC138549, MSTD, MTBT1, MTBT2, PPND, PPP1R103, tau

Western blot (Supportive data in Antibodypedia)

Immunohistochemistry (Data presented on provider website)

Other assay (Supportive data in Antibodypedia)

Antibody Data

Product number

823902

Provider

BioLegend

Proper citation

BioLegend Cat#823902, RRID:AB_2715848

Product name

Purified anti-1N Tau

Antibody type

Monoclonal

Reactivity

Human

Host

Mouse

Antibody clone number

4H5.B9

Vial size

25 µL

Concentration

1.00 mg/mL

Storage

2°C-8°C

Handling

Ambient RT

Western blot

Supportive validation

Submitted by

BioLegend (provider)

Main image

Experimental details

Western blot of purified anti-1N Tau antibody (clone 4H5.B9). Lane 1: Molecular weight marker; Lane 2: 20 µg of human brain lysate; Lane 3: 0.1 µg of recombinant human Tau ladder protein; Lane 4: 0.1 µg of recombinant human 1N3R Tau protein. The blot was incubated with 1 µg/mL of the primary antibody overnight at 4°C, followed by incubation with HRP labeled goat anti-mouse IgG (Cat. No. 405306). Enhanced chemiluminescence was used as the detection system.

Other assay

Supportive validation

Submitted by

BioLegend (provider)

Main image

Experimental details

Western Blot analysis showing 1N Tau specificity: Lane 1: tau protein ladder; Lane 2: 1N3R tau recombinant protein isoform; Lane3: 0N3R tau recombinant protein isoform; Lane 4: 2N3R tau recombinant protein isoform.