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Learn710080
antibody from Invitrogen Antibodies
Targeting: MAPT
DDPAC, FLJ31424, FTDP-17, MAPTL, MGC138549, MSTD, MTBT1, MTBT2, PPND, PPP1R103, tau
Western blot
ELISAAntibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [1]
- Immunocytochemistry [1]
- Other assay [1]
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- Product number
- 710080 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- Tau Recombinant Polyclonal Antibody (6HCLC)
- Antibody type
- Polyclonal
- Antigen
- Recombinant full-length protein
- Reactivity
- Human
- Host
- Rabbit
- Isotype
- IgG
- Antibody clone number
- 6HCLC
- Vial size
- 100 µg
- Concentration
- 0.5 mg/mL
- Storage
- Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of Tau in Jurkat whole cell extract (80 µg) using a Tau Recombinant Rabbit Polyclonal Antibody (Product # 710080) at a dilution of 5 µg/mL. Detection was performed using an HRP-conjugated Goat anti-Rabbit secondary antibody at a dilution of 1:2000 followed by chemiluminescence (ECL). Results show a band at ~68 kDa.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunofluorescence analysis of Tau (red) in human glutamate neurons derived from iPSCs. The cells were fixed with 4% paraformaldehyde for 10 minutes, permeabilized with 0.2% Triton X-100 in PBS for 10 minutes, and blocked with 5% donkey serum in PBS for 15 minutes at room temperature. Cells were stained with a Tau Recombinant Rabbit Polyclonal Antibody (Product # 710080) at 1:500 and a Map2 monoclonal antibody (Product # MA5-12826) diluted at 1:1000 in 5% donkey serum overnight at 4°C, and then incubated with a Goat anti-Rabbit IgG (H+L) secondary antibody, Alexa Fluor 546 conjugate (Product # A-11010, red) and Goat anti-Mouse IgG (H+L), Alexa Fluor® 488 conjugate (Product # A28175, green) at a dilution of 1:1000 for 1 hour at room temperature. Note: Data courtesy of Data Exchange Program.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Indirect ELISA analysis of endogenous Tau in Jurkat whole cell lysate (300 ng/well) using a Tau Recombinant Rabbit Polyclonal Antibody (Product # 710080) followed by detection using an HRP-conjugated Goat anti-Rabbit secondary antibody (Product # G-21234) and TMB (Product # SB01) as a substrate.
