- PA5-27287 - Invitrogen Antibodies MAPT antibody

Submitted references Isolation and characterization of antibody fragment selective for human Alzheimer's disease brain-derived tau variants.

Venkataraman L, He P, Schulz P, Sierks MR
Neurobiology of aging 2020 Oct;94:7-14

Comparison of (18)F-T807 and (18)F-THK5117 PET in a Mouse Model of Tau Pathology.

Brendel M, Yousefi BH, Blume T, Herz M, Focke C, Deussing M, Peters F, Lindner S, von Ungern-Sternberg B, Drzezga A, Bartenstein P, Haass C, Okamura N, Herms J, Yakushev I, Rominger A
Frontiers in aging neuroscience 2018;10:174

Eicosanoyl-5-hydroxytryptamide (EHT) prevents Alzheimer's disease-related cognitive and electrophysiological impairments in mice exposed to elevated concentrations of oligomeric beta-amyloid.

Asam K, Staniszewski A, Zhang H, Melideo SL, Mazzeo A, Voronkov M, Huber KL, Pérez E, Stock M, Stock JB, Arancio O, Nicholls RE
PloS one 2017;12(12):e0189413

No comments: Submit comment

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Western blot analysis of Tau using A) 30 µg SK-N-SH whole cell lysate (B) 30 µg IMR32 whole cell lysate and C) 30 µg SK-N-AS whole cell lysate. Samples were loaded onto a 10% SDS-PAGE gel and probed with a Tau polyclonal antibody (Product # PA5-27287) at a dilution of 1:500.

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Western blot analysis of Tau using 50 µg of mouse brain lysate. Samples were loaded onto a 10% SDS-PAGE gel and probed with a Tau polyclonal antibody (Product # PA5-27287) at a dilution of 1:3000.

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Western Blot analysis of Tau was performed by separating 30 µg of various whole cell extracts by 7.5% SDS-PAGE. Proteins were transferred to a membrane and probed with a Tau Polyclonal Antibody (Product # PA5-27287) at a dilution of 1:1000 and a HRP-conjugated anti-rabbit IgG secondary antibody.

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Western Blot analysis of Tau was performed by separating 50 µg of Mouse tissue extracts by 7.5% SDS-PAGE. Proteins were transferred to a membrane and probed with a Tau Polyclonal Antibody (Product # PA5-27287) at a dilution of 1:3000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Western blot was performed using Anti-Tau Polyclonal Antibody (Product # PA5-27287) and 40-80 kDa bands corresponding to Tau were observed across in Mouse and Rat Brain but not in Mouse Kidney or Mouse and Rat Lung. Tissue extracts (30 µg lysate) of Mouse Brain (Lane 1), Rat Brain (Lane 2), Mouse Kidney (Lane 3), Mouse Lung (Lane 4) or Rat Lung (Lane 5) were electrophoresed using NuPAGE™ 10% Bis-Tris Protein Gel (Product # NP0301BOX). Resolved proteins were then transferred onto a Nitrocellulose membrane (Product # IB23001) by iBlot® 2 Dry Blotting System (Product # IB21001). The blot was probed with the primary antibody (1:500) and detected by chemiluminescence with Goat anti-Rabbit IgG (H+L) Superclonal™ Recombinant Secondary Antibody, HRP (Product # A27036,1:4000) using the iBright FL 1000 (Product # A32752). Chemiluminescent detection was performed using Novex® ECL Chemiluminescent Substrate Reagent Kit (Product # WP20005).

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Immunohistochemistry (Paraffin) analysis of Tau was performed in paraffin-embedded mouse eye tissue using Green: Tau Polyclonal Antibody (Product # PA5-29610) at a dilution of 1:500. Red: beta Tubulin 3/ Tuj1, a cytoskeleton marker, stained by beta Tubulin 3/ Tuj1 antibody diluted at 1:500. Blue: Fluoroshield with DAPI. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min.

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Immunohistochemistry (Paraffin) analysis of Tau was performed in paraffin-embedded mouse eye tissue using Green: Tau Polyclonal Antibody (Product # PA5-29610) at a dilution of 1:500. Red: beta Tubulin 3/ Tuj1, a cytoskeleton marker, stained by beta Tubulin 3/ Tuj1 antibody diluted at 1:500. Blue: Fluoroshield with DAPI. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min.

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Immunohistochemistry (Paraffin) analysis of Tau was performed in paraffin-embedded mouse cerebellum tissue using Tau Polyclonal Antibody (Product # PA5-27287) at a dilution of 1:500. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min.

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: FIGURE 6 (A) PET-Immunohistochemistry correlation of 18 F-T807 (blue diamonds) and 18 F-THK5117 (yellow circles) SUVR with %tau/area to PA5-27287 antibody staining. (B) 3-dimensional brainstem acquisition of PA5-27287 staining and (C) zoomed image indicating automatically detected tau positive cell somata. (D) 2-dimensional whole brain overview shows tau-positive cells predominantly in the brainstem, and lower amounts in the neocortex. Data derive from N = 5 P301S mice.

PA5-27287

Antibody data