PA5-50956

antibody from Invitrogen Antibodies

Targeting: MRC2 CD280, CLEC13E, ENDO180, KIAA0709

Western blot (Supportive data in Antibodypedia)

Immunohistochemistry (Recommended by provider)

Other assay (Supportive data in Antibodypedia)

Antibody Data

Product number

PA5-50956

Provider

Invitrogen Antibodies

Product name

MRC2 Polyclonal Antibody

Antibody type

Polyclonal

Antigen

Synthetic peptide

Description

MRC2 Polyclonal Antibody detects endogenous levels of MRC2 protein.

Reactivity

Human, Mouse, Rat

Host

Rabbit

Isotype

IgG

Vial size

100 µL

Concentration

1 mg/mL

Storage

-20° C, Avoid Freeze/Thaw Cycles

References

Submitted references

MRC2 Promotes Proliferation and Inhibits Apoptosis of Diabetic Nephropathy.

Li L, Chen X, Zhang H, Wang M, Lu W
Analytical cellular pathology (Amsterdam) 2021;2021:6619870

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Transplantation-Induced Ischemia-Reperfusion Injury Modulates Antigen Presentation by Donor Renal CD11c(+)F4/80(+) Macrophages through IL-1R8 Regulation.

Aiello S, Podestà MA, Rodriguez-Ordonez PY, Pezzuto F, Azzollini N, Solini S, Carrara C, Todeschini M, Casiraghi F, Noris M, Remuzzi G, Benigni A
Journal of the American Society of Nephrology : JASN 2020 Mar;31(3):517-531

Western blot

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Western blot analysis of MRC2 in 293 cells. Samples were probed using MRC2 Polyclonal Antibody (Product # PA5-50956).

Other assay

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Figure 1 The expression of MRC2 measured in the peripheral blood of 21 healthy subjects and 21 patients with DN detected by qRT-PCR increased in patients with DN (a). MRC2 expression in cell and animal models of DN and MRC2 silencing. The MRC2 expression in MMCs cultured under low-glucose and high-glucose conditions was detected using western blotting (b) and qRT-PCR (c). The MRC2 expression in the renal tissues of normal and DN mice was detected using western blotting (d) and qRT-PCR (e). The MRC2 expression in MMCs transfected with the negative control (si-NC) and siRNAs (si-MRC2 1 and si-MRC2 2) was detected using western blotting (f) and qRT-PCR (g). *** P < 0.001 vs. healthy group. ** P < 0.01, *** P < 0.001 vs. control group.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Figure 2 The renal tissues of normal and DN mice were collected for H&E staining and immunohistochemistry analysis (a), and immunofluorescence was used to detect the expression of MRC2 in MMCs cultured under low-glucose and high-glucose conditions (b). *** P < 0.001 vs. control group.