PA5-101901

antibody from Invitrogen Antibodies

Targeting: SLC8B1 FLJ22233, NCKX6, NCLX, SLC24A6

Western blot (Supportive data in Antibodypedia)

Immunocytochemistry (Supportive data in Antibodypedia)

Other assay (Supportive data in Antibodypedia)

Antibody Data

Product number

PA5-101901

Provider

Invitrogen Antibodies

Product name

SLC8B1 Polyclonal Antibody

Antibody type

Polyclonal

Antigen

Synthetic peptide

Description

Antibody detects endogenous levels of total SLC24A6.

Reactivity

Human

Host

Rabbit

Isotype

IgG

Vial size

100 µL

Concentration

1 mg/mL

Storage

-20°C

References

Submitted references

Calcium Signaling and Mitochondrial Function in Presenilin 2 Knock-Out Mice: Looking for Any Loss-of-Function Phenotype Related to Alzheimer's Disease.

Rossi A, Galla L, Gomiero C, Zentilin L, Giacca M, Giorgio V, Calì T, Pozzan T, Greotti E, Pizzo P
Cells 2021 Jan 21;10(2)

Western blot

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Western blot analysis of SLC8B1 in MCF-7 cells (left lane: treated with the antigen-specific peptide). Samples were incubated with SLC8B1 polyclonal antibody (Product # PA5-101901).

Immunocytochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunofluorescent analysis of SLC8B1 in MCF7. Samples were fixed with paraformaldehyde, permeabilized with 0.1% Triton X-100, blocked with 10% serum (45 min at 25°C) incubated with SLC8B1 polyclonal antibody (Product # PA5-101901) using a dilution of 1:200 (1 hr, 37°C), and followed by goat anti-rabbit IgG Alexa Fluor 594 at a dilution of 1:600.

Other assay

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Figure 3 The lack of PS2 does not affect the mitochondrial mass. Representative Western blots of different mitochondrial protein levels in WT and PS2-/- cortical neurons. Each sample was run in duplicate. The corresponding scatter plot represents the mean values +- SEM. Values were first normalized to their internal housekeeping proteins (as indicated) and then to those of WT samples. ( A - G ) Representative Western blot and quantification of: ( A ) mitochondrial Ca 2+ uniporter (MCU) protein levels of 6 independent cultures from WT mice and 5 independent cultures from PS2-/- mice; ( B ) NCLX protein levels of 4 independent cultures from WT mice and from PS2-/- mice; ( C ) TOM20 protein levels of 4 independent cultures from WT mice and from PS2-/- mice; ( D ) MFN2 protein levels of 14 independent cultures from WT mice and 8 independent cultures from PS2-/- mice; ( E ) MFN1 protein levels of 4 independent cultures from WT mice and from PS2-/- mice; ( F ) cyclophilin D (cycl D) protein levels of 4 independent cultures from WT mice and 3 independent cultures from PS2-/- mice. ( G ) The bar graph shows the mean values +- SEM of Nonyl Acridine Orange (NAO) fluorescence (arbitrary units) from 4 independent cultures from WT mice and 3 independent cultures from PS2-/- mice, as a percentage of WT.