Antibody data
- Antibody Data
- Antigen structure
- References [6]
- Comments [0]
- Validations
- Immunocytochemistry [1]
- Flow cytometry [1]
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- Product number
- MAB2405 - Provider product page
- Provider
- R&D Systems
- Product name
- Mouse Integrin beta 1/CD29 Antibody
- Antibody type
- Monoclonal
- Description
- Protein A or G purified from hybridoma culture supernatant. Detects mouse Integrin beta 1/CD29 in direct ELISAs and Western blots. In Western blots, no cross-reactivity with recombinant human Integrin beta 1, recombinant mouse (rm) Integrin beta 2, rmIntegrin beta 3, rmIntegrin beta 4, or rmIntegrin beta 6 is observed.
- Reactivity
- Mouse
- Host
- Rat
- Conjugate
- Unconjugated
- Antigen sequence
P09055
- Isotype
- IgG
- Antibody clone number
- 265917
- Vial size
- 100 ug
- Concentration
- LYOPH
- Storage
- Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Submitted references Bush-like integrin filament networks associated with hyaloid vasculature in murine neonate eyes.
Histochemical characteristics of regressing vessels in the hyaloid vascular system of neonatal mice: Novel implication for vascular atrophy.
Autologous transplantation of adipose-derived stem cells improves functional recovery of skeletal muscle without direct participation in new myofiber formation.
Antibody-based targeting of alternatively spliced tissue factor: a new approach to impede the primary growth and spread of pancreatic ductal adenocarcinoma.
Crawling phagocytes recruited in the brain vasculature after pertussis toxin exposure through IL6, ICAM1 and ITGαM.
Absence of keratin 8 confers a paradoxical microflora-dependent resistance to apoptosis in the colon.
Iwanaga T, Nio-Kobayashi J, Takahashi-Iwanaga H
Biomedical research (Tokyo, Japan) 2019;40(2):79-85
Biomedical research (Tokyo, Japan) 2019;40(2):79-85
Histochemical characteristics of regressing vessels in the hyaloid vascular system of neonatal mice: Novel implication for vascular atrophy.
Kishimoto A, Kimura S, Nio-Kobayashi J, Takahashi-Iwanaga H, Park AM, Iwanaga T
Experimental eye research 2018 Jul;172:1-9
Experimental eye research 2018 Jul;172:1-9
Autologous transplantation of adipose-derived stem cells improves functional recovery of skeletal muscle without direct participation in new myofiber formation.
Gorecka A, Salemi S, Haralampieva D, Moalli F, Stroka D, Candinas D, Eberli D, Brügger L
Stem cell research & therapy 2018 Jul 17;9(1):195
Stem cell research & therapy 2018 Jul 17;9(1):195
Antibody-based targeting of alternatively spliced tissue factor: a new approach to impede the primary growth and spread of pancreatic ductal adenocarcinoma.
Unruh D, Ünlü B, Lewis CS, Qi X, Chu Z, Sturm R, Keil R, Ahmad SA, Sovershaev T, Adam M, Van Dreden P, Woodhams BJ, Ramchandani D, Weber GF, Rak JW, Wolberg AS, Mackman N, Versteeg HH, Bogdanov VY
Oncotarget 2016 May 3;7(18):25264-75
Oncotarget 2016 May 3;7(18):25264-75
Crawling phagocytes recruited in the brain vasculature after pertussis toxin exposure through IL6, ICAM1 and ITGαM.
Richard JF, Roy M, Audoy-Rémus J, Tremblay P, Vallières L
Brain pathology (Zurich, Switzerland) 2011 Nov;21(6):661-71
Brain pathology (Zurich, Switzerland) 2011 Nov;21(6):661-71
Absence of keratin 8 confers a paradoxical microflora-dependent resistance to apoptosis in the colon.
Habtezion A, Toivola DM, Asghar MN, Kronmal GS, Brooks JD, Butcher EC, Omary MB
Proceedings of the National Academy of Sciences of the United States of America 2011 Jan 25;108(4):1445-50
Proceedings of the National Academy of Sciences of the United States of America 2011 Jan 25;108(4):1445-50
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Supportive validation
- Submitted by
- R&D Systems (provider)
- Main image
- Experimental details
- Integrin beta 1/CD29 in L-929 Mouse Cell Line. Integrin beta 1/CD29 was detected in immersion fixed L-929 mouse fibroblast cell line using Rat Anti-Mouse Integrin beta 1/CD29 Monoclonal Antibody (Catalog # MAB2405) at 10 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Rat IgG Secondary Antibody (yellow; Catalog # NL013) and counterstained with DAPI (blue). View our protocol for Fluorescent ICC Staining of Cells on Coverslips.
Supportive validation
- Submitted by
- R&D Systems (provider)
- Main image
- Experimental details
- Detection of Integrin beta 1/CD29 in B16-F1 Mouse Cell Line by Flow Cytometry. B16-F1 mouse melanoma cell line was stained with Rat Anti-Mouse Integrin beta 1/CD29 Monoclonal Antibody (Catalog # MAB2405, filled histogram) or isotype control antibody (Catalog # MAB006, open histogram), followed by Phycoerythrin-conjugated Anti-Rat IgG Secondary Antibody (Catalog # F0105B).