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Western blot
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- Antibody Data
- Antigen structure
- References [6]
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- Validations
- Western blot [2]
- Immunohistochemistry [1]
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- Product number
- AF2405 - Provider product page
- Provider
- R&D Systems
- Product name
- Mouse/Rat Integrin beta 1/CD29 Antibody
- Antibody type
- Polyclonal
- Description
- Antigen Affinity-purified. Detects mouse Integrin beta 1/CD29 in direct ELISAs. Detects mosue and rat Integrin beta 1/CD29 in Western blots. In direct ELISAs, approximately 5% cross-reactivity with recombinant human (rh) Integrin beta 1 and rhIntegrin beta 3 is observed and less than 1% cross-reactivity with recombinant mouse (rm) Integrin beta 2, rmIntegrin beta 4, and rmIntegrin beta 6 is observed.
- Reactivity
- Mouse, Rat
- Host
- Goat
- Conjugate
- Unconjugated
- Antigen sequence
P09055- Isotype
- IgG
- Vial size
- 100 ug
- Concentration
- LYOPH
- Storage
- Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Submitted references Cell-matrix signals specify bone endothelial cells during developmental osteogenesis.
Exon skipping restores dystrophin expression, but fails to prevent disease progression in later stage dystrophic dko mice.
Methamphetamine-associated cleavage of the synaptic adhesion molecule intercellular adhesion molecule-5.
Loss of beta1 integrin in mouse fibroblasts results in resistance to skin scleroderma in a mouse model.
Galectin-7 in the control of epidermal homeostasis after injury.
Tetraspanin CD81 is required for the alpha v beta5-integrin-dependent particle-binding step of RPE phagocytosis.
Langen UH, Pitulescu ME, Kim JM, Enriquez-Gasca R, Sivaraj KK, Kusumbe AP, Singh A, Di Russo J, Bixel MG, Zhou B, Sorokin L, Vaquerizas JM, Adams RH
Nature cell biology 2017 Mar;19(3):189-201
Nature cell biology 2017 Mar;19(3):189-201
Exon skipping restores dystrophin expression, but fails to prevent disease progression in later stage dystrophic dko mice.
Wu B, Cloer C, Lu P, Milazi S, Shaban M, Shah SN, Marston-Poe L, Moulton HM, Lu QL
Gene therapy 2014 Sep;21(9):785-93
Gene therapy 2014 Sep;21(9):785-93
Methamphetamine-associated cleavage of the synaptic adhesion molecule intercellular adhesion molecule-5.
Conant K, Lonskaya I, Szklarczyk A, Krall C, Steiner J, Maguire-Zeiss K, Lim ST
Journal of neurochemistry 2011 Aug;118(4):521-32
Journal of neurochemistry 2011 Aug;118(4):521-32
Loss of beta1 integrin in mouse fibroblasts results in resistance to skin scleroderma in a mouse model.
Liu S, Kapoor M, Denton CP, Abraham DJ, Leask A
Arthritis and rheumatism 2009 Sep;60(9):2817-21
Arthritis and rheumatism 2009 Sep;60(9):2817-21
Galectin-7 in the control of epidermal homeostasis after injury.
Gendronneau G, Sidhu SS, Delacour D, Dang T, Calonne C, Houzelstein D, Magnaldo T, Poirier F
Molecular biology of the cell 2008 Dec;19(12):5541-9
Molecular biology of the cell 2008 Dec;19(12):5541-9
Tetraspanin CD81 is required for the alpha v beta5-integrin-dependent particle-binding step of RPE phagocytosis.
Chang Y, Finnemann SC
Journal of cell science 2007 Sep 1;120(Pt 17):3053-63
Journal of cell science 2007 Sep 1;120(Pt 17):3053-63
No comments: Submit comment
Supportive validation
- Submitted by
- R&D Systems (provider)
- Main image
- Experimental details
- Detection of Mouse and Rat Integrin beta 1/CD29 by Simple WesternTM. Simple Western lane view shows lysates of L-929 mouse fibroblast cell line, NIH-3T3 mouse embryonic fibroblast cell line, PC-12 rat adrenal pheochromocytoma cell line, and Rat-2 rat embryonic fibroblast cell line, loaded at 0.2 mg/mL. A specific band was detected for Integrin beta 1/CD29 at approximately 153-174 kDa (as indicated) using 10 µg/mL of Goat Anti-Mouse/Rat Integrin beta 1/CD29 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF2405) followed by 1:50 dilution of HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF109). This experiment was conducted under reducing conditions and using the 12-230 kDa separation system.
- Submitted by
- R&D Systems (provider)
- Main image
- Experimental details
- Detection of Mouse and Rat Integrin beta 1/CD29 by Western Blot. Western blot shows lysates of L-929 mouse fibroblast cell line, NIH-3T3 mouse embryonic fibroblast cell line, PC-3 human prostate cancer cell line, Rat-2 rat embryonic fibroblast cell line, and RAW 264.7 mouse monocyte/macrophage cell line. PVDF membrane was probed with 1 µg/mL of Goat Anti-Mouse/Rat Integrin beta 1/CD29 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF2405) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF017). A specific band was detected for Integrin beta 1/CD29 at approximately 110-140 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
Supportive validation
- Submitted by
- R&D Systems (provider)
- Main image
- Experimental details
- Orexin B, p38 alpha , & Integrin beta 1 in Mouse Brainstem. Orexin B, p38 alpha , and Integrin beta 1 were detected in perfusion fixed frozen sections of mouse brainstem using Human Orexin B Monoclonal Antibody (red; Catalog # MAB734), Human/Mouse/Rat p38 alpha Antigen Affinity-purified Polyclonal Antibody (green; Catalog # AF8691), and Mouse/Rat Integrin beta 1 Antigen Affinity-purified Polyclonal Antibody (blue; Catalog # AF2405). Tissue was incubated with primary antibodies overnight at 4 °C. Tissue was stained using NorthernLights™ 493-conjugated Anti-Rabbit IgG Secondary Antibody (green; Catalog # NL006), NorthernLights 557-conjugated Anti-Mouse IgG Secondary Antibody (red; Catalog # NL007), and NorthernLights 637-conjugated Anti-Goat IgG Secondary Antibody (blue; Catalog # NL002). The image of Integrin beta 1 is pseudo-colored for presentation. View our protocol for Fluorescent IHC Staining of Frozen Tissue Sections.
