M00772-1

antibody from Boster Biological Technology

Targeting: ITGB1 CD29, FNRB, GPIIA, MDF2, MSK12

Western blot (Data presented on provider website)

Immunohistochemistry (Supportive data in Antibodypedia)

Antibody Data

Product number

M00772-1

Provider

Boster Biological Technology

Product name

Anti-Integrin beta 1 ITGB1 Rabbit Monoclonal Antibody

Antibody type

Monoclonal

Description

Monoclonal antibody for Integrin beta 1/ITGB1 detection. Host: Rabbit.Size: 100ug/vial. Tested applications: IHC, WB. Reactive species: Human, Mouse, Rat Integrin beta 1/ITGB1 information: Molecular Weight: 88415 MW; Subcellular Localization: Cell membrane ; Single-pass type I membrane protein . Cell projection, invadopodium membrane ; Single-pass type I membrane protein . Cell projection, ruffle membrane ; Single- pass type I membrane protein . Recycling endosome. Melanosome. Cleavage furrow. Cell projection, lamellipodium. Cell projection, ruffle. Isoform 2 does not localize to focal adhesions. Highly enriched in stage I melanosomes. Located on plasma membrane of neuroblastoma NMB7 cells. In a lung cancer cell line, in prometaphase and metaphase, localizes diffusely at the membrane and in a few intracellular vesicles. In early telophase, detected mainly on the matrix-facing side of the cells. By mid- telophase, concentrated to the ingressing cleavage furrow, mainly to the basal side of the furrow. In late telophase, concentrated to the extending protrusions formed at the opposite ends of the spreading daughter cells, in vesicles at th

Reactivity

Human, Mouse, Rat

Host

Rabbit

Antibody clone number

DDI-9

Vial size

100ug/vial

Concentration

0.5-1mg/ml, actual concentration vary by lot. Use suggested dilution ratio to decide dilution procedure.

Storage

At -20°C for one year. Avoid repeated freezing and thawing.

References

Submitted references

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Huang Y, Jia X, Bai K, Gong X, Fan Y
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Rat bone marrow derived mesenchymal progenitor cells support mouse ES cell growth and germ-like cell differentiation.

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Inhibitory effect of mesenchymal stem cells on lymphocyte proliferation.

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Asian journal of andrology 2007 Sep;9(5):659-67

Immunohistochemistry

Supportive validation

Submitted by

Boster Biological Technology (provider)

Main image

Experimental details

Immunohistochemical analysis of paraffin-embedded human breast cancer, using Integrin beta 1 Antibody(M00772-1)ITGB1 was detected in paraffin-embedded tissue section. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1ug/ml rabbit anti-ITGB1 Antibody (M00772-1)overnight at 4

Additional image