LS-C815147

antibody from LSBio

Targeting: ITGB1 CD29, FNRB, GPIIA, MDF2, MSK12

Western blot (Enhanced validation)

Immunoprecipitation (Supportive data in Antibodypedia)

Antibody Data

Product number

LS-C815147

Provider

LSBio

Product name

ITGB1 / Integrin Beta 1 / CD29 Antibody (Preservative Free) LS-C815147

Antibody type

Polyclonal

Description

Protein A affinity chromatography

Reactivity

Mouse

Host

Rabbit

Isotype

IgG

Storage

Store at 2°C to 8°C for up to 1 month. Aliquot and store at -20°C to -80°C for up to 1 year. Avoid freeze/thaw cycles.

Western blot

Enhanced validation

Submitted by

LSBio (provider)

Enhanced method

Genetic validation

Main image

Experimental details

Anti-ITGB1 rabbit polyclonal antibody at 1:500 dilution. Lane A: Raw264.7 Whole Cell Lysate. Lane B: Mouse heart tissue lysate. Lane C: Mouse kidney tissue lysate. Lane D: Mouse spleen tissue lysate. Lysates/proteins at 30 ug per lane. Secondary: Goat Anti-Rabbit IgG (H+L)/HRP at 1/10000 dilution. Developed using the ECL technique. Performed under reducing conditions. Predicted band size: 88 kDa.

Immunoprecipitation

Supportive validation

Submitted by

LSBio (provider)

Enhanced method

Genetic validation

Main image

Experimental details

LCP1 was immunoprecipitated using: Lane A: 0.5 mg Jurkat Whole Cell Lysate. 4 uL anti-LCP1 rabbit polyclonal antibody and 60 ug of Immunomagnetic beads Protein A/G. Primary antibody: Anti-LCP1 rabbit polyclonal antibody, at 1:100 dilution. Secondary antibody: Goat Anti-Rabbit IgG (H+L)/HRP at 1/10000 dilution. Developed using the ECL technique. Performed under reducing conditions. Predicted band size: 70 kDa. Observed band size: 70 kDa.

Supportive validation

Submitted by

LSBio (provider)

Main image

Experimental details

LCP1 was immunoprecipitated using: Lane A: 0.5 mg Jurkat Whole Cell Lysate. 4 uL anti-LCP1 rabbit polyclonal antibody and 60 ug of Immunomagnetic beads Protein A/G. Primary antibody: Anti-LCP1 rabbit polyclonal antibody, at 1:100 dilution. Secondary antibody: Goat Anti-Rabbit IgG (H+L)/HRP at 1/10000 dilution. Developed using the ECL technique. Performed under reducing conditions. Predicted band size: 70 kDa. Observed band size: 70 kDa.

Supportive validation

Submitted by

LSBio (provider)

Main image

Experimental details

LCP1 was immunoprecipitated using: Lane A: 0.5 mg Jurkat Whole Cell Lysate. 4 uL anti-LCP1 rabbit polyclonal antibody and 60 ug of Immunomagnetic beads Protein A/G. Primary antibody: Anti-LCP1 rabbit polyclonal antibody, at 1:100 dilution. Secondary antibody: Goat Anti-Rabbit IgG (H+L)/HRP at 1/10000 dilution. Developed using the ECL technique. Performed under reducing conditions. Predicted band size: 70 kDa. Observed band size: 70 kDa.