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Western blot
ImmunocytochemistryAntibody data
- Antibody Data
- Antigen structure
- References [4]
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- Validations
- Immunocytochemistry [1]
- Flow cytometry [1]
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- Product number
- MAB1778 - Provider product page
- Provider
- R&D Systems
- Product name
- Human Integrin beta 1/CD29 Antibody
- Antibody type
- Monoclonal
- Description
- Protein A or G purified from hybridoma culture supernatant. Detects human Integrin beta 1/CD29 in direct ELISAs and Western blots.
- Reactivity
- Human
- Host
- Mouse
- Conjugate
- Unconjugated
- Isotype
- IgG
- Antibody clone number
- 4B7R
- Vial size
- 100 ug
- Concentration
- LYOPH
- Storage
- Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Submitted references Bacterial genotoxins promote inside-out integrin β1 activation, formation of focal adhesion complexes and cell spreading.
Nanoparticle biointerfacing by platelet membrane cloaking.
Proinflammatory and antimicrobial nitric oxide in gingival fluid of diabetic patients with periodontal disease.
Aberration of CCR7 CD8 memory T cells from patients with systemic lupus erythematosus: an inducer of T helper type 2 bias of CD4 T cells.
Levi L, Toyooka T, Patarroyo M, Frisan T
PloS one 2015;10(4):e0124119
PloS one 2015;10(4):e0124119
Nanoparticle biointerfacing by platelet membrane cloaking.
Hu CM, Fang RH, Wang KC, Luk BT, Thamphiwatana S, Dehaini D, Nguyen P, Angsantikul P, Wen CH, Kroll AV, Carpenter C, Ramesh M, Qu V, Patel SH, Zhu J, Shi W, Hofman FM, Chen TC, Gao W, Zhang K, Chien S, Zhang L
Nature 2015 Oct 1;526(7571):118-21
Nature 2015 Oct 1;526(7571):118-21
Proinflammatory and antimicrobial nitric oxide in gingival fluid of diabetic patients with periodontal disease.
Skaleric U, Gaspirc B, McCartney-Francis N, Masera A, Wahl SM
Infection and immunity 2006 Dec;74(12):7010-3
Infection and immunity 2006 Dec;74(12):7010-3
Aberration of CCR7 CD8 memory T cells from patients with systemic lupus erythematosus: an inducer of T helper type 2 bias of CD4 T cells.
Sen Y, Chunsong H, Baojun H, Linjie Z, Qun L, San J, Qiuping Z, Junyan L, Zhang X, Jinquan T
Immunology 2004 Jun;112(2):274-89
Immunology 2004 Jun;112(2):274-89
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Supportive validation
- Submitted by
- R&D Systems (provider)
- Main image
- Experimental details
- Integrin beta 1/CD29 in MG-63 Human Cell Line. Integrin beta 1/CD29 was detected in immersion fixed MG-63 human osteosarcoma cell line using Mouse Anti-Human Integrin beta 1/CD29 Monoclonal Anti-body (Catalog # MAB1778) at 10 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Mouse IgG Secondary Anti-body (yellow; Catalog # NL007) and counter-stained with DAPI (blue). View our protocol for Fluorescent ICC Staining of Cells on Coverslips.
Supportive validation
- Submitted by
- R&D Systems (provider)
- Main image
- Experimental details
- Detection of Integrin beta 1/CD29 in Human PBMCs by Flow Cytometry. Human peripheral blood mononuclear cells (PBMCs) were stained with Mouse Anti-Human Integrin beta 1/CD29 Monoclonal Antibody (Catalog # MAB1778, filled histogram) or isotype control antibody (Catalog # MAB002, open histogram), followed by Phycoerythrin-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # F0102B).
