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Immunocytochemistry
ImmunoprecipitationAntibody data
- Antibody Data
- Antigen structure
- References [2]
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- Validations
- Immunocytochemistry [1]
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- Product number
- MAB17782 - Provider product page
- Provider
- R&D Systems
- Product name
- Human Integrin beta 1/CD29 Antibody
- Antibody type
- Monoclonal
- Description
- Protein A or G purified from hybridoma culture supernatant. Detects human Integrin beta 1/CD29. Reacts with calcium-dependent epitopes on the human Integrin beta 1 subunit. This antibody activates Integrin beta 1-dependent binding to extracellular matrix substrates. The use of binding buffer containing 1 mM Ca+2 is recommended.
- Reactivity
- Human
- Host
- Mouse
- Conjugate
- Unconjugated
- Isotype
- IgG
- Antibody clone number
- P4G11
- Vial size
- 100 ug
- Concentration
- LYOPH
- Storage
- Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Submitted references The tetraspanin CD9 modulates epidermal growth factor receptor signaling in cancer cells.
Integrin-mediated host cell invasion by type 1-piliated uropathogenic Escherichia coli.
Murayama Y, Shinomura Y, Oritani K, Miyagawa J, Yoshida H, Nishida M, Katsube F, Shiraga M, Miyazaki T, Nakamoto T, Tsutsui S, Tamura S, Higashiyama S, Shimomura I, Hayashi N
Journal of cellular physiology 2008 Jul;216(1):135-43
Journal of cellular physiology 2008 Jul;216(1):135-43
Integrin-mediated host cell invasion by type 1-piliated uropathogenic Escherichia coli.
Eto DS, Jones TA, Sundsbak JL, Mulvey MA
PLoS pathogens 2007 Jul;3(7):e100
PLoS pathogens 2007 Jul;3(7):e100
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Supportive validation
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- R&D Systems (provider)
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- Experimental details
- Integrin beta 1/CD29 in HeLa Human Cell Line. Integrin beta 1/CD29 was detected in immersion fixed HeLa human cervical epithelial carcinoma cell line using Mouse Anti-Human Integrin beta 1/CD29 Monoclonal Antibody (Catalog # MAB17782) at 25 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Mouse IgG Secondary Antibody (red; Catalog # NL007) and counterstained with DAPI (blue). Specific staining was localized to cytoplasm and cell surfaces. View our protocol for Fluorescent ICC Staining of Cells on Coverslips.
