Antibody data
- Antibody Data
- Antigen structure
- References [1]
- Comments [0]
- Validations
- Western blot [1]
- Immunocytochemistry [1]
Submit
Validation data
Reference
Comment
Report error
- Product number
- AF8047 - Provider product page
- Provider
- R&D Systems
- Product name
- Human/Mouse/Rat EEA1 Antibody
- Antibody type
- Polyclonal
- Description
- Antigen Affinity-purified. Detects human, mouse, and rat EEA1 in Western blots.
- Reactivity
- Human, Mouse, Rat
- Host
- Sheep
- Conjugate
- Unconjugated
- Antigen sequence
Q15075
- Isotype
- IgG
- Vial size
- 100 ug
- Concentration
- LYOPH
- Storage
- Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Submitted references EGFL7 enhances surface expression of integrin α(5)β(1) to promote angiogenesis in malignant brain tumors.
Dudvarski Stanković N, Bicker F, Keller S, Jones DT, Harter PN, Kienzle A, Gillmann C, Arnold P, Golebiewska A, Keunen O, Giese A, von Deimling A, Bäuerle T, Niclou SP, Mittelbronn M, Ye W, Pfister SM, Schmidt MHH
EMBO molecular medicine 2018 Sep;10(9)
EMBO molecular medicine 2018 Sep;10(9)
No comments: Submit comment
Supportive validation
- Submitted by
- R&D Systems (provider)
- Main image
- Experimental details
- Detection of Human, Mouse, and Rat EEA1 by Western Blot. Western blot shows lysates of JAR human choriocarcinoma cell line, Hepa 1-6 mouse hepatoma cell line, and Sheep Anti-Rat-2 rat embryonic fibroblast cell line. PVDF membrane was probed with 1 µg/mL of Sheep Anti-Human/Mouse/Rat EEA1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF8047) followed by HRP-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # HAF016). A specific band was detected for EEA1 at approximately 170 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
Supportive validation
- Submitted by
- R&D Systems (provider)
- Main image
- Experimental details
- EEA1 in HeLa Human Cell Line. EEA1 was detected in immersion fixed HeLa human cervical epithelial carcinoma cell line using Sheep Anti-Human/Mouse/Rat EEA1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF8047) at 1.7 and 5 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Sheep IgG Secondary Antibody (red; Catalog # NL010) and counterstained with DAPI (blue). Specific staining was localized to early endosomes. View our protocol for Fluorescent ICC Staining of Cells on Coverslips.