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- Antibody Data
- Antigen structure
- References [7]
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- Immunocytochemistry [1]
- Other assay [4]
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- Product number
- 14-9114-82 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- EEA1 Monoclonal Antibody (1G11), eBioscience™
- Antibody type
- Monoclonal
- Antigen
- Other
- Description
- Description: The monoclonal antibody 1G11 recognizes human, mouse and rat early endosome antigen 1 (EEA1). EEA1 is a hydrophilic peripheral membrane protein found in the cytosol and present on early endosome membranes. EEA1 is a dimer that is localized to early endosomes via a cysteine-rich zinc-finger-like FYVE domain. EEA1 interacts with Rab5-GTP, syntaxin 6 and syntaxin 13 (SNARES). EEA1 is required for endocytic membrane docking and fusion, which allows for the recycling of receptors from the plasma membrane or their delivery to lysosomes for degradation. In neurons, early endosomes are involved in the recycling of neurotransmitter receptors. Autoantibodies to EEA1 have been found in patients with neurological deficits which result in enhanced excitatory synaptic transmission. Applications Reported: This 1G11 antibody has been reported for use in western blotting, immunohistochemical staining of formalin-fixed paraffin embedded tissue sections, microscopy, ELISA, and immunocytochemistry. Applications Tested: This 1G11 antibody has been tested by immunocytochemistry of fixed and permeabilized HeLa cells and can be used at less than or equal to 1 µg/mL. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest. Purity: Greater than 90%, as determined by SDS-PAGE. Aggregation: Less than 10%, as determined by HPLC. Filtration: 0.2 µm post-manufacturing filtered.
- Reactivity
- Human, Mouse, Rat
- Host
- Mouse
- Isotype
- IgG
- Antibody clone number
- 1G11
- Vial size
- 100 µg
- Concentration
- 0.5 mg/mL
- Storage
- 4° C
Submitted references Erlotinib Promotes Ligand-Induced EGFR Degradation in 3D but Not 2D Cultures of Pancreatic Ductal Adenocarcinoma Cells.
Bi-allelic VPS16 variants limit HOPS/CORVET levels and cause a mucopolysaccharidosis-like disease.
Merging organoid and organ-on-a-chip technology to generate complex multi-layer tissue models in a human retina-on-a-chip platform.
Characterization of early endosome antigen 1 in neural tissues.
The Rab5 effector EEA1 interacts directly with syntaxin-6.
Early endosome antigen. 1: An autoantigen associated with neurological diseases.
EEA1, an early endosome-associated protein. EEA1 is a conserved alpha-helical peripheral membrane protein flanked by cysteine "fingers" and contains a calmodulin-binding IQ motif.
Betriu N, Andreeva A, Semino CE
Cancers 2021 Sep 7;13(18)
Cancers 2021 Sep 7;13(18)
Bi-allelic VPS16 variants limit HOPS/CORVET levels and cause a mucopolysaccharidosis-like disease.
Sofou K, Meier K, Sanderson LE, Kaminski D, Montoliu-Gaya L, Samuelsson E, Blomqvist M, Agholme L, Gärtner J, Mühlhausen C, Darin N, Barakat TS, Schlotawa L, van Ham T, Asin Cayuela J, Sterky FH
EMBO molecular medicine 2021 May 7;13(5):e13376
EMBO molecular medicine 2021 May 7;13(5):e13376
Merging organoid and organ-on-a-chip technology to generate complex multi-layer tissue models in a human retina-on-a-chip platform.
Achberger K, Probst C, Haderspeck J, Bolz S, Rogal J, Chuchuy J, Nikolova M, Cora V, Antkowiak L, Haq W, Shen N, Schenke-Layland K, Ueffing M, Liebau S, Loskill P
eLife 2019 Aug 27;8
eLife 2019 Aug 27;8
Characterization of early endosome antigen 1 in neural tissues.
Selak S, Braun JE, Fritzler MJ
Biochemical and biophysical research communications 2004 Oct 29;323(4):1334-42
Biochemical and biophysical research communications 2004 Oct 29;323(4):1334-42
The Rab5 effector EEA1 interacts directly with syntaxin-6.
Simonsen A, Gaullier JM, D'Arrigo A, Stenmark H
The Journal of biological chemistry 1999 Oct 8;274(41):28857-60
The Journal of biological chemistry 1999 Oct 8;274(41):28857-60
Early endosome antigen. 1: An autoantigen associated with neurological diseases.
Selak S, Chan EK, Schoenroth L, Senécal JL, Fritzler MJ
Journal of investigative medicine : the official publication of the American Federation for Clinical Research 1999 Jul;47(6):311-8
Journal of investigative medicine : the official publication of the American Federation for Clinical Research 1999 Jul;47(6):311-8
EEA1, an early endosome-associated protein. EEA1 is a conserved alpha-helical peripheral membrane protein flanked by cysteine "fingers" and contains a calmodulin-binding IQ motif.
Mu FT, Callaghan JM, Steele-Mortimer O, Stenmark H, Parton RG, Campbell PL, McCluskey J, Yeo JP, Tock EP, Toh BH
The Journal of biological chemistry 1995 Jun 2;270(22):13503-11
The Journal of biological chemistry 1995 Jun 2;270(22):13503-11
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunocytochemistry of fixed and permeabilized HeLa cells using 1 µg/mL of Mouse IgG1 K Isotype Control Purified (left) or 1 µg/mL of Anti-Early Endosome Antigen 1 (EEA1) Purified (right) followed by F (ab')2 Anti-Mouse IgG eFluor® 570.Nuclei are stained with DAPI.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- 4 Figure Analysis of transferrin uptake and trafficking in fibroblasts A, B Analysis of the early endosome marker EEA1 in fibroblasts transduced with control or VPS16 -expressing lentivirus by confocal microscopy. (A) Representative confocal micrographs. Scale bar, 20 mum. (B) Quantification of EEA1-stained puncta number and intensities (ca 80 cells analyzed for each of n = 3 biological replicates). C Analysis of transferrin uptake in fibroblasts transduced with the indicated lentiviruses. Representative confocal micrographs of fibroblasts fed with Alexa488-conjugated Transferrin for 30 min. Scale bar, 10 mum. D Quantifications of the number and fluorescence intensities of intracellular Transferrin puncta ( n = 28-30 fields from three independent experiments). Colored horizontal bars indicate median values and whiskers 5 and 95 percentiles. E Immunoblot for Transferrin receptor (TfR) levels in the indicated fibroblasts under basal and serum-starved conditions. F Summary quantification of Transferrin receptor levels in non-starved fibroblasts transduced with the indicated lentiviruses, normalized to actin (ACTB), and expressed as % of controls ( n = 6 biological replicates). G, H Analysis of intracellular Transferrin trafficking. Fibroblasts fed with fluorescently labeled Transferrin were co-stained with LysoTracker (G) or an antibody against LAMP2 (H). Representative images (left) of fibroblasts transduced with control virus, with magnified areas demonstrating co-localized pu
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Figure 9 Colocalization analysis of the EGFR and EEA1 (early endosomes) in cells incubated with EGF, erlotinib or both in 2D cultures. EGFR (red) and EEA1 (green) immunofluorescence counterstained with DAPI (grey) in ( a ) PANC-1; ( b ) BxPC-3 and ( c ) hNDF cells. Scale bars represent 10 um; ( d ) Manders' colocalization coefficients showing the proportion of EGFR overlapping with EEA1; ( e ) Manders' colocalization coefficients showing the proportion of EEA1 overlapping with EGFR. Statistical differences are indicated as * for p < 0.05, ** for p < 0.01, *** for p < 0.001 and **** for p < 0.0001.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Figure 11 Immunofluorescence of EGFR and EEA1 in ( a ) BxPC-3 cells and ( b ) hNDF incubated with EGF, erlotinib or both in 0.15% RAD16-I 3D cultures. One representative Z plane is shown. Scale bars represent 10 um. White arrows show EGFR colocalizing with early endosomes.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Figure 7--figure supplement 1. Phagocytosis assay in dish cultured hiPSC-derived RPE. hiPSC-RPE were incubated with bovine photoreceptor outer segments (POS) and after 2 hr stained positive for endosomal marker EEA1 (green, left panel) and rhodopsin (RHOD, green, right panel). Scale bars: 40 um. Blue: DAPI.
