RQ4105

antibody from NSJ Bioreagents

Targeting: EEA1 ZFYVE2

Antibody data

Submit

Validation data

Product number: RQ4105 - Provider product page
Provider: NSJ Bioreagents
Product name: EEA1 Antibody
Antibody type: Polyclonal
Description: This highly specific EEA1 antibody is suitable for use in Western blot/Immunohistochemistry/Immunofluorescence applications with human, mouse and rat samples.
Reactivity: Human, Mouse, Rat
Host: Rabbit
Conjugate: Unconjugated
Vial size: 100 ug
Concentration: 0.5mg/ml if reconstituted with 0.2ml sterile DI water
Storage: After reconstitution, the EEA1 antibody can be stored for up to one month at 4oC. For long-term, aliquot and store at -20oC. Avoid repeated freezing and thawing.

EEA1 protein structure - RQ4105 shown in red.

Supportive validation

Submitted by: NSJ Bioreagents (provider)
Main image
Experimental details: Western blot testing of human 1) HeLa, 2) placenta, 3) COLO320, 4) 22RV1 and 5) A549 lysate with EEA1 antibody at 0.5ug/ml. Predicted molecular weight ~162 kDa.

Supportive validation

Submitted by: NSJ Bioreagents (provider)
Main image
Experimental details: Western blot testing of rat 1) spleen, 2) thymus, 3) brain, 4) lung and mouse 5) spleen, 6) brain, 7) HEPA1-6 and 8) NIH3T3 lysate with EEA1 antibody at 0.5ug/ml. Predicted molecular weight ~162 kDa.

Supportive validation

Submitted by: NSJ Bioreagents (provider)
Main image
Experimental details: Western blot testing of human 1) HeLa, 2) placenta, 3) COLO320, 4) 22RV1 and 5) A549 lysate with EEA1 antibody at 0.5ug/ml. Predicted molecular weight ~162 kDa.

Supportive validation

Submitted by: NSJ Bioreagents (provider)
Main image
Experimental details: Western blot testing of rat 1) spleen, 2) thymus, 3) brain, 4) lung and mouse 5) spleen, 6) brain, 7) HEPA1-6 and 8) NIH3T3 lysate with EEA1 antibody at 0.5ug/ml. Predicted molecular weight ~162 kDa.

Supportive validation

Submitted by: NSJ Bioreagents (provider)
Main image
Experimental details: Western blot testing of human 1) RT4, 2) HaCaT, 3) HepG2, 4) SiHa, 5) K562 and 6) MCF7 cell lysate with EEA1 antibody at 0.5ug/ml. Predicted molecular weight ~162 kDa.

Supportive validation

Submitted by: NSJ Bioreagents (provider)
Main image
Experimental details: Western blot testing of 1) rat brain, 2) rat lung, 3) mouse brain, 4) mouse kidney and 5) mouse lung tissue lysate with EEA1 antibody at 0.5ug/ml. Predicted molecular weight ~162 kDa.